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Chainia Penicillin V Acylase: Strain Characteristics, Enzyme Immobilization, and Kinetic Studies

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Abstract.

Aerobic cultures of an actinomycete were found to produce penicillin V acylase (PVA) (PA, EC-3.5.1.11) extracellularly. The presence of L-2-3 diamino-propionic acid in cell wall and formation of sclerotia on culture media led to its identification as Chainia, a sclerotial Streptomyces. Partially purified acylase was adsorbed on kieselguhr and entrapped in polyacrylamide gel. The immobilized preparation proved effective with respect to retention of enzyme and enzyme activity even after 15 successful cycles. The pH optimum for crude enzyme was in the range of pH 7.5–8.0, and for the (NH4)2 SO4 fraction it was pH 8.5. The immobilized enzyme showed maximal activity at pH 9.5. The optimum temperature for acylase activity was at 55°C. The crude enzyme, ammonium sulfate fraction, and immobilized enzyme showed K m value for penicillin V of 6.13 mM, 14.3 mM, and 17.1 mM, respectively.

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Authors and Affiliations

  1. Molecular Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi-110067, India , , , , , , IN

    Sanjay Chauhan

  2. Genetic Engineering Unit, Center for Biotechnology, Jawaharlal Nehru University, New Delhi-110067, India , , , , , , IN

    Anuradha Nichkawade

  3. Microbiological Research and Development Laboratories, Alembic Chemical Works Co. Ltd, Baroda-390002, India , , , , , , IN

    M.R.S. Iyengar

  4. Department of Microbiology and Biotechnology Center, M.S. University of Baroda, Baroda-390002, India , , , , , , IN

    Bharat B. Chattoo

Authors
  1. Sanjay Chauhan
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  2. Anuradha Nichkawade
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  3. M.R.S. Iyengar
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  4. Bharat B. Chattoo
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Received: 11 December 1997 / Accepted: 9 April 1998

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Chauhan, S., Nichkawade, A., Iyengar, M. et al. Chainia Penicillin V Acylase: Strain Characteristics, Enzyme Immobilization, and Kinetic Studies. Curr Microbiol 37, 186–190 (1998). https://doi.org/10.1007/s002849900361

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  • DOI: https://doi.org/10.1007/s002849900361

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