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Cloning and Expression of a Bacillus thuringiensis (L1-2) Gene Encoding a Crystal Protein Active Against Glossina morsitans morsitans and Chilo partellus

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Abstract.

A local isolate of Bacillus thuringiensis, designated L1-2, that is toxic to Chilo partellus was found to be toxic to the adult tsetse fly, Glossina morsitans morsitans. The δ-endotoxin crystals derived from the isolate gave a major protein band with a molecular weight of Mr 130,000–140,000 on denaturing polyacrylamide gel electrophoresis. The sequence of the cloned gene was found to be similar to that of the B. thuringiensis subsp. kurstaki HD-73 cryIA(c) gene, having one amino acid difference at position 148 and four additional DNA differences.

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Authors and Affiliations

  1. The International Center of Insect Physiology and Ecology (ICIPE), P.O. Box 30772, Nairobi, Kenya , , , , , , KE

    Edward O. Omolo & Ellie O. Osir

  2. Microbiology Department, University of Cape Town, Private Bag, Rondebosch 7700, South Africa , , , , , , ZA

    M. Diane James & Jennifer A. Thomson

Authors
  1. Edward O. Omolo
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  2. M. Diane James
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  3. Ellie O. Osir
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  4. Jennifer A. Thomson
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Received: 29 June 1996 / Accepted: 1 August 1996

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Omolo, E., James, M., Osir, E. et al. Cloning and Expression of a Bacillus thuringiensis (L1-2) Gene Encoding a Crystal Protein Active Against Glossina morsitans morsitans and Chilo partellus . Curr Microbiol 34 , 118 –121 (1997). https://doi.org/10.1007/s002849900154

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  • DOI: https://doi.org/10.1007/s002849900154

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