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The Influence of Probiotics in Reducing Cisplatin-Induced Toxicity in Zebrafish (Danio rerio)

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Abstract

In this work, the effects of probiotic supplementation on cisplatin toxicity in zebrafish (Danio rerio) were examined. For this study, adult female zebrafish were given cisplatin (G2), the probiotic, Bacillus megaterium (G3), and cisplatin+B. megaterium (G4) for 30 days, in addition to the control (G1). In order to investigate changes in antioxidative enzymes, ROS production, and histological changes after treatment, the intestines and ovaries were excised. The levels of lipid peroxidation, glutathione peroxidase, glutathione reductase, catalase, and superoxide dismutase were found to be significantly higher in the cisplatin group than in the control group in both the intestine and the ovaries. Administration of the probiotic and cisplatin effectively reversed this damage. Histopathological analyses showed that the cisplatin group had much more damage than the control group and that probiotic+cisplatin treatment significantly cured these damages. It opens the door to probiotics being combined with cancer-related drugs, which may be a more efficient approach for minimizing side effects. The underlying molecular mechanisms of probiotics must be further investigated.

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Acknowledgements

The authors wish to thank Dr. Subramanyam Dasari, Indiana University and Dr. Mallikarjuna Nimgampalle, IISER Tirupati, India for their help in revising this manuscript. We are also thankful to Dr. Buddolla’s Institute of Life Sciences for their technical support.

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Authors and Affiliations

  1. Dr. Buddolla’s Institute of Life Sciences, Tirupati, 517503, India

    Kusuma Kandati, Ankireddy Seshadri Reddy & Buddolla Viswanath

  2. Department of Biotechnology, Sri Padmavati Mahila Visvavidyalayam, Tirupati, 517 502, India

    Kusuma Kandati & John Sushma Nannepaga

Authors
  1. Kusuma Kandati
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  2. Ankireddy Seshadri Reddy
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  3. John Sushma Nannepaga
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  4. Buddolla Viswanath
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Contributions

KK: Methodology, Data curation, Formal analysis, Writing – original draft. ASR: Data curation, Investigation, Formal analysis. JSN: Data curation, Investigation, Formal analysis, Supervision. BV: Conceptualization, Investigation, Supervision, Validation, Funding acquisition, Project administration, Writing—review & editing.

Corresponding authors

Correspondence to John Sushma Nannepaga or Buddolla Viswanath.

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Conflict of interest

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Ethical Approval

All experiments have been conducted as per the guidelines of the Institutional Animal Ethics Committee, Dr. Buddolla’s Institute of Life Sciences, Tirupati, India (Registration No. 2172/PO/Re/S/2022/CPCSEA dated 9th June 2022

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Kandati, K., Reddy, A.S., Nannepaga, J.S. et al. The Influence of Probiotics in Reducing Cisplatin-Induced Toxicity in Zebrafish (Danio rerio). Curr Microbiol 80, 109 (2023). https://doi.org/10.1007/s00284-023-03203-5

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  • DOI: https://doi.org/10.1007/s00284-023-03203-5

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