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Whole Genome, Functional Annotation and Comparative Genomics of Plant Growth-Promoting Bacteria Pseudomonas aeruginosa (NG61) with Potential Application in Agro-Industry

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Abstract

A plant growth-promoting Rhizobacteria (PGPR) Pseudomonas aeruginosa (NG61) isolated from rhizosphere of Sunflower plant. The isolate was identified by 16S rRNA gene sequencing (Accession no. MK455763). NG61 showed various plant growth promotion and biocontrol activities like, Phosphate solubilisation, Nitrogen fixation, Ammonia production, IAA production, siderophore production, HCN production. The whole genome sequence of Pseudomonas aeruginosa (NG61) was reported and analysed. The estimated genome size was 6537180 bp with 66.18% of G+C content. The genome encoded 6186 protein-coding genes, 6252 genes were predicted, 66RNA genes. Phylogenetic tree showed that the P. aeruginosa( NG61) was closely related to P.aeruginosa strain DSM 50071. The annotated draft genome has been deposited at the NCBI database under the accession number PRJNA707114 BioProject and BioSample: SAMN18174979. The analysis of genome sequence of P. aeruginosa (NG61) showed various genes encoding plant growth promotion and biocontrol activities.

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Data Availability

The data of whole genome was saved as FASTQ files and deposited into National Center for Biotechnology Information under accession numbers of BioProject PRJNA707114 and BioSample: SAMN18174979.

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Acknowledgements

The authors are thankful to DIC MHRD New Delhi for the financial Assistance.

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The authors are thankful to DIC MHRD New Delhi for the financial Assistance.

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  1. Department of Botany, Savitribai Phule Pune University, Pune, MH, 411007, India

    Tejal Rikame & Mahesh Borde

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  1. Tejal Rikame
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TR performed the In vitro plant growth-promoting activities. MB carried out genome analysis and written and approved the final manuscript.

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Correspondence to Mahesh Borde.

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Rikame, T., Borde, M. Whole Genome, Functional Annotation and Comparative Genomics of Plant Growth-Promoting Bacteria Pseudomonas aeruginosa (NG61) with Potential Application in Agro-Industry. Curr Microbiol 79, 169 (2022). https://doi.org/10.1007/s00284-022-02845-1

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