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Response of Milk Performance, Rumen and Hindgut Microbiome to Dietary Supplementation with Aspergillus oryzae Fermentation Extracts in Dairy Cows

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Abstract

Fungal additives had beneficial effects on milk performance in dairy cows. Previous studies investigating the effects of fungal additives mainly focused on the rumen, such influences on the hindgut remain limited. This study aimed to investigate the effects of Aspergillus oryzae fermentation extracts (AOE) on the milk performance and microbiome in the rumen and hindgut using 16S rRNA gene sequencing. Twenty lactating multiparous Holstein cows were randomly assigned to control and treatment (5 g AOE per cow per day). The results showed that AOE increased the milk yield, milk protein and lactose concentration, but did not affect the milk fat concentration. Feeding AOE did not affect the ruminal VFA pattern, pH, NH3-N, and microbial cell protein production, but decreased lipopolysaccharide concentration and tended to decrease lactate concentration. The addition of AOE increased the fecal pH and the proportions of propionate, isovalerate and valerate, and decreased the acetate to propionate ratio. PCoA analysis showed that AOE did not affect the overall ruminal bacterial population composition. Only three genera changed slightly in relative abundance. In the feces, PCoA analysis showed that AOE changed the bacterial population composition. Feeding AOE increased the relative abundances of Ruminococcaceae UCG-010 and unclassified Clostridiales vadinBB60 group, and decreased Christensenellaceae R-7 group, unclassified Muribaculaceae, Prevotellaceae UCG-001 and Romboutsia. Spearman correlation showed unclassified Clostridiales vadinBB60 group was positively correlated with propionate proportion. Overall, we present that AOE not only functioned in rumen, but also in hindgut. The hindgut microbiome changes might play an important role in the milk performance improvement of dairy cows.

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Data Availability

The raw data of Illumina MiSeq sequences were submitted to the Sequence Read Archive (SRA; http://www.ncbi.nlm.nih.gov/Traces/sra/) under Accession No. SRP217364.

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Funding

This work was supported by the National Key Research and Development Program of China (No. 2018YFD0501600).

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Authors and Affiliations

  1. Laboratory of Gastrointestinal Microbiology, Jiangsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China

    Jiyou Zhang, Wei Jin, Fei Xie & Shengyong Mao

  2. Department of Animal Sciences, University of Florida, Gainesville, 32611, USA

    Yun Jiang

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  1. Jiyou Zhang
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  2. Wei Jin
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Contributions

JZ conducted the animal experiment, samplings, chemical analyses. WJ performed the statistical analysis and drafted the manuscript. YJ revised critically this manuscript. FX analyzed the microbial sequencing data. SM conceived this study.

Corresponding author

Correspondence to Wei Jin.

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The authors declare no conflict of interest.

Ethical Approval

The animal experimental design and procedures of this study were supported by the Animal Care and Use Committee of Nanjing Agricultural University following the requirements of the Regulations for the Administration of Affairs Concerning Experimental Animals (The State Science and Technology Commission of P. R. China, 1988. No. SYXK (Su) 2015-0656).

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Zhang, J., Jin, W., Jiang, Y. et al. Response of Milk Performance, Rumen and Hindgut Microbiome to Dietary Supplementation with Aspergillus oryzae Fermentation Extracts in Dairy Cows. Curr Microbiol 79, 113 (2022). https://doi.org/10.1007/s00284-022-02790-z

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