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Arabiibacter massiliensis gen. nov. sp. nov., New Anaerobic Bacterium Isolated from the Human Gut

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Abstract

Using microbial culturomics, we were able to isolate strain Marseille-P3078 from a stool sample of a healthy 50-year-old Saudi Arabian woman. To this end, we used taxonogenomics that combines phenotypic, biochemical and genomic analyses, to describe this bacterium. Cells from strain Marseille-P3078 are anaerobic and Gram-negative rods that are motile and unable to sporulate. Its genome size is 3,377,914-bp-long with a 66.33 mol% G + C content. Based on its phenotypic and genomic features, including a 94.6% 16S rRNA similarity with Paraeggerthella hongkongensis strain JCM 14552, its closest phylogenetic neighbor withstanding in nomenclature, we propose that strain Marseille-P3078T (= CSUR P3078 = DSM 104007) is the representative strain of a new genus for which we propose the name Arabiibacter massiliensis gen. nov., sp. nov.

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Acknowledgements

The authors thank Enora Tomei for her technical assistance, Ludivine Brechard for sequencing the genome and Aurélia Caputo for depositing the genome sequence in Genbank.

Funding

The study was funded by the Mediterranee Infection foundation and the French Agence Nationale de la Recherche under reference Investissements d’Avenir Méditerranée Infection 10-IAHU-03.

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Authors and Affiliations

  1. UMR VITROME, Aix Marseille Univ, IRD, AP-HM, SSA, Marseille, France

    Cheikh Ibrahima Lo, Awa Diop, Florence Fenollar & Pierre-Edouard Fournier

  2. IHU-Méditerranée Infection, Marseille, France

    Cheikh Ibrahima Lo, Sory Ibrahima Traore, Awa Diop, Melhem Bilen, Jean-Christophe Lagier, Didier Raoult, Florence Fenollar & Pierre-Edouard Fournier

  3. UMR MEPHI, Aix Marseille Univ, IRD, AP-HM, 19-21 Boulevard Jean Moulin, 13385, Marseille cedex 05, France

    Sory Ibrahima Traore, Melhem Bilen & Jean-Christophe Lagier

  4. Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia

    Esam Ibraheem Azhar, Muhammad Yasir & Didier Raoult

  5. Department of Medical Laboratory Technology, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia

    Esam Ibraheem Azhar, Fehmida Bibi & Asif Jiman-Fatani

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  1. Cheikh Ibrahima Lo
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Contributions

Conceptualization, DR and PEF; methodology, PEF; software, AD and MB; validation, DR, JCL, FF and PEF; formal analysis, SIT, AD, MB, and CIL; investigation, SIT, and EIA; resources, EIA, FB, AJF, and MY; data curation, CIL and SIT; writing-original draft preparation, CIL and SIT writing-review and editing, CIL, FF, PEF; supervision, PEF and FF; project administration, PEF and DR; funding acquisition, DR All authors have read and agreed to the published version of the manuscript.

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Correspondence to Pierre-Edouard Fournier.

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Lo, C.I., Traore, S.I., Diop, A. et al. Arabiibacter massiliensis gen. nov. sp. nov., New Anaerobic Bacterium Isolated from the Human Gut. Curr Microbiol 79, 47 (2022). https://doi.org/10.1007/s00284-021-02739-8

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