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Pseudaminobacter soli sp. nov., Isolated from Paddy Soil Contaminated with Heavy Metals

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Abstract

A Gram stain-negative, aerobic, rod-shaped strain, designated HC19T, was isolated from heavy metals contaminated paddy soil. The 16S rRNA gene-based phylogenetic analysis indicated that strain HC19T belonged to the genus Pseudaminobacter, and shared 97.0% 16S rRNA gene sequence similarity with P. manganicus JH-7T, and less than 97% similarities with other type strains belonging to the genus. The major cellular fatty acids were C19:0 cyclo ω8c (55.0%) and C18: 1ω7c (18.7%). The major quinone was ubiquinone Q-10. The major polar lipids were phosphatidylmonomethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and phosphatidylethanolamine. The average nucleotide identity and digital DNA–DNA hybridization values between the genomes of HC19T and P. manganicus JH-7T were 68.0% and 22%, respectively. The G+C content of the genomic DNA was 63.3 mol%. On the basis of phenotypic, chemotaxonomic, and genotypic data, strain HC19T is considered as a novel species in the genus Pseudaminobacter, for which the name Pseudaminobacter. soli sp. nov. is proposed. The type strain is HC19T (= KCTC 82870T = CCTCC AB 2021107T).

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Acknowledgements

The study was supported by the Natural Science Foundation of China (Grant Nos. 31870092 and 31970108).

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  1. Kaiyun Zhang and Shitong Tang have contributed to the work equally.

Authors and Affiliations

  1. Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China

    Kaiyun Zhang, Yinhu Jiang, Fuyin Zhang, Jiguo Qiu & Jian He

  2. Jiangsu Key Laboratory for Organic Waste Utilization, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, 210095, China

    Shitong Tang & Jun Zhang

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  1. Kaiyun Zhang
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Contributions

JQ, JZ, KZ and JH carried out the concepts. JL and ST participated in the research and analyzed the data. YJ and FZ provided assistances for literature search and data acquisition. KZ drafted the manuscript. JQ, JZ and JH performed manuscript review. All authors read and approved the final manuscript.

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Correspondence to Jiguo Qiu.

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The research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. All the authors declare that they have no conflict of interest.

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The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences are MW664033.2 (Sanger sequencing), while for the whole genome of strains HC19T are JAGWCR000000000.

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Zhang, K., Tang, S., Jiang, Y. et al. Pseudaminobacter soli sp. nov., Isolated from Paddy Soil Contaminated with Heavy Metals. Curr Microbiol 79, 19 (2022). https://doi.org/10.1007/s00284-021-02717-0

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