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Evolution of Subfamily I.1 Lipases in Pseudomonas aeruginosa

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Abstract

The gram-negative Pseudomonas aeruginosa is an opportunistic human pathogen that contains two different types of strains: the “classical” and the “outlier”. In the “classical” strain, its bacterial subfamily I.1 lipases, such as LipA and LipC in P. aeruginosa PAO1, play critical roles in its pathogenicity. However, less is known about the subfamily I.1 lipases in the “outlier” strain, nor the evolution paths of those lipases in both types of P. aeruginosa strains. Our genome-scale investigation on I.1 lipases across different bacterial strains demonstrates the presence of one LipA-like and one new type of I.1 lipase (LipC2) in those “outlier” strains. The related genomic islands analyses further suggest that the LipC counterpart gene in the “outlier” strain was lost by gene truncation. In addition, the evolutionary analyses also indicates the horizontal LipC2 gene transfer from other gammaproteobacterial species, as well as the horizontal LipA gene transfer between two different phyla, both suggesting that the gene transfer of bacterial I.1 lipases might occur in different taxonomical levels. Our results not only provide an evidence to understand the pathogenicity among different P. aeruginosa strains, but add to the knowledge of I.1 lipase evolution in bacteria.

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Acknowledgements

We appreciate Dr. Wang for his constructive comments on this manuscript.

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  1. State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China

    Zhenghong Zhang & Xuehong Zhang

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ZZ and XZ conceived and designed the experiments. ZZ performed experiments, analyzed the experimental data, and drafted the manuscript. XZ revised the manuscript. All authors read and approved the final manuscript.

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Correspondence to Zhenghong Zhang.

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Zhang, Z., Zhang, X. Evolution of Subfamily I.1 Lipases in Pseudomonas aeruginosa. Curr Microbiol 78, 3494–3504 (2021). https://doi.org/10.1007/s00284-021-02589-4

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