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Evaluation of the Carbon Dioxide Production by Fungi Under Different Growing Conditions

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Abstract

Production of carbon dioxide, as one of the ultimate products of fungal metabolism, can be used to quantify and measure their metabolic rate under different conditions, thus aiding in finding the optimal substrate and environment for cultivation of wood-destroying fungi. This study is focused on species Pleurotus ostreatus and Ganoderma lucidum,. These species are also cultivated for mycorestoration as well as their medicinal and nutritional value. To quantify their metabolical rate on various substrates (agar medium, wood chips, rye straw), multiple custom-built airtight chambers were equipped with CO2 probes (GMP 343, Vaisala, Finland) to measure the production of carbon dioxide. The highest values were measured during the primordial production on rye straw substrate, with the average values of 1.09 g CO2 kg−1 (substrate) h−1. These values varied significantly between various substrates, fungal species and development stages.

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Acknowledgements

The study was supported by grants of the Slovak Research and Development Agency No. APVV-17-0644, APVV-16-0325 and grants of the Slovak Grant Agency KEGA No. 006TU Z-4/2019 and VEGA 1/0367/16.

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Authors and Affiliations

  1. Department of Integrated Forest and Landscape Protection, Faculty of Forestry, Technical University in Zvolen, T.G. Masaryka 20, 96001, Zvolen, Slovak Republic

    Martin Pavlík, Peter Fleischer, Peter Fleischer Jr. & Miriama Šuleková

  2. Slovak Academy of Sciences, Institute of Forest Ecology, 96001, Zvolen, Slovak Republic

    Peter Fleischer Jr.

  3. Faculty of Medicine, Comenius University, 81372, Bratislava, Slovak Republic

    Martin Pavlík Jr.

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  1. Martin Pavlík
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  2. Peter Fleischer
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  4. Martin Pavlík Jr.
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  5. Miriama Šuleková
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Contributions

MP prepared substrates, inoculated fungi, wrote paper. PF Sr. run the experiments, interpreted the measurements. PF Jr. designed the experiment, run the statistical analysis, disccused the results. MP Jr. prepared fungal cultures and substrates, linguistic text editing. MŠ working with samples, laboratory measurements.

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Correspondence to Martin Pavlík.

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Pavlík, M., Fleischer, P., Fleischer, P. et al. Evaluation of the Carbon Dioxide Production by Fungi Under Different Growing Conditions. Curr Microbiol 77, 2374–2384 (2020). https://doi.org/10.1007/s00284-020-02033-z

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