Abstract
A fungus, designated as strain SS2 able to degrade aliphatic polyesters, poly(3-hydroxybutyrate) (PHB) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), was isolated from soil. Strain SS2 was identified through rDNA gene sequencing and showed maximum closeness to Penicillium oxalicum. The newly isolated P. oxalicum strain SS2 had completely degraded PHB and PHBV both in emulsion and films form within 36–48 h at 30 °C. Furthermore, P. oxalicum SS2 degraded PHB and PHBV films in soil environment in lab-built soil microcosms within 1 week. The polymer films were evaluated for changes after degradation through scanning electron microscopy (SEM), nuclear magnetic resonance (NMR), differential scanning calorimetry (DSC) and Fourier Transform Infrared spectroscopy (FTIR). The PHBV depolymerase enzyme was purified to homogeneity through column chromatography and molecular mass was found approximately 36 kDa. The depolymerase was stable over a wide range of temperature (15–60 °C) and pH (3.0–8.0) with optimum 40 °C and pH 5.0. The enzyme activity was significantly affected by various metal ions and surfactants. The enzyme activity was strongly enhanced in the presence of divalent cationic metal Cu2+ while inhibited by Zn2+ and non-polar detergents Tween 20 and Tween 60. Finally, it is concluded that P. oxalicum strain SS2 has profound degradation capabilities, and can be applied for the treatment of plastic-contaminated environments.
Similar content being viewed by others
References
Nadhman A, Hasan F, Shah Z, Hameed A, Shah AA (2012) Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) depolymerase from Aspergillus sp. NA-25. Appl Biochem Microbiol 48(5):531–536
Tokiwa Y, Calabia BP (2004) Degradation of microbial polyesters. Biotechnol Lett 26(15):1181–1189
Reid BJ, Jones KC, Semple KT (2000) Bioavailability of persistent organic pollutants in soils and sediments—a perspective on mechanisms, consequences and assessment. Environ Pollut 108(1):103–112
Singh R, Paul D, Jain RK (2006) Biofilms: implications in bioremediation. Trends Microbiol 14(9):389–397
Schroll R, Becher HH, Rfler UD, Gayler S, Grundmann S, Hartmann HP, Ruossandju R (2006) Quantifying the effect of soil moisture on the aerobic microbial mineralization of selected pesticides in different soils. Environ Sci Technol 40:3305–3312
Grundmann S, Fus R, Schmid M, Laschinger M, Ruth B, Schulin R, Munch JC, Schroll R (2007) Application of microbial hot spots enhances pesticide degradation in soils. Chemosphere 68:511–517
Fang H, Dong B, Yan H, Tang F, Yu Y (2010) Characterization of a bacterial strain capable of degrading DDT congeners and its use in bioremediation of contaminated soil. J Hazard Mater 184:281–289
Włóka D, Placek A, Rorat A, Smol M, Kacprzak M (2017) The evaluation of polycyclic aromatic hydrocarbons (PAHs) biodegradation kinetics in soil amended with organic fertilizers and bulking agents. Ecotoxicol Environ Saf 145:161–168
Megharaj M, Ramakrishnan B, Venkateswarlu K, Sethunathan N, Naidu R (2011) Bioremediation approaches for organic pollutants: a critical perspective. Environ Int 37:1362–1375
Krueger MC, Harms H, Schlosser D (2015) Prospects for microbiological solutions to environmental pollution with plastics. Appl Microbiol Biotechnol 99:8857–8874
Mergaert J, Webb A, Anderson C, Wouters A, Swings J (1993) Microbial degradation of poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in soils. Appl Environ Microbiol 59:3233–3238
Liu H, Gao Z, Hu X, Wang Z, Su T, Yang L, Yan S (2017) Blending modification of PHBV/PCL and its biodegradation by Pseudomonas mendocina. J Polym Environ 25:156–164
Shah AA, Hasan F, Hameed A, Ahmed S (2007) Isolation and characterization of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) degrading bacteria and purification of PHBV depolymerase from newly isolated Bacillus sp. AF3. Int Biodeterior Biodegrad 60:109–115
Shah AA, Hasan F, Hameed A (2010) Degradation of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) by a newly isolated Actinomadura sp. AF-555, from soil. Int Biodeterior Biodegrad 64:281–285
Rong IH, Baxter AP (2006) The South African National Collection of Fungi: celebrating a centenary 1905–2005. Stud Mycol 55:1–12
Zhang YJ, Zhang S, Liu XZ, Wen HA, Wang M (2010) A simple method of genomic DNA extraction suitable for analysis of bulk fungal strains. Lett Appl Microbiol 51:114–118
Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729
Shah Z, Krumhulz L, Aktas DF, Hasan F, Khattak M, Shah AA (2013) Degradation of polyester polyurethane by newly isolated Pseudomonas aeruginosa strain MZA-85 and analysis of degradation products by GC–MS. Int Biodeterior Biodegrad 77:114–122
Maliszewska-Kordybach B, Smreczak B (2000) Ecotoxicological activity of soils polluted with polycyclic aromatic hydrocarbons (PAHs)-effect on plants. Environ Technol 21:1099–1110
Placek A, Grobelak A, Kacprzak M (2016) Improving the phytoremediation of heavy metals contaminated soil by use of sewage sludge. Int J Phytoremediat 18:605–618
Yamamoto-tamura K, Hiradate S, Watanabe T, Koitabashi M, Sameshima-yamashita Y, Yarimizu T, Kitamoto H (2015) Contribution of soil esterase to biodegradation of aliphatic polyester agricultural mulch film in cultivated soils. AMB Express 5:10
Park HJ, Jeon JH, Kang SG, Lee JH, Lee SA, Kim HK (2007) Functional expression and refolding of new alkaline esterase, EM2L8 from deep-sea sediment metagenome. Protein Expr Purif 52:340–347
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680
Kasuya KI, Ishii N, Inoue Y, Yazawa K, Tagaya T, Yotsumoto T, Nagai KJI (2009) Characterization of a mesophilic aliphatic–aromatic copolyester-degrading fungus. Polym Degrad Stab 94:1190–1196
Lee KM, Gimore DF, Huss MJ (2005) Fungal degradation of the bioplastic PHB (poly-3-hydroxy-butyric acid). J Polym Environ 13:213–219
Hoshino A, Tsuji M, Fukuda K, Nonagase M, Sawada H, Kimura M (2002) Changes in molecular structure of biodegradable plastics during degradation in soils estimated by FT-IR and NMR. Soil Sci Plant Nutr 48:469–473
Llauro-darricades MF, Bensemra N, Guyot A, Petiaud R (1989) 13C and 1H NMR analysis of structural defects in PVC: application to the production of defects at various polymerization stages. Makromol Chem Macromol Sym 29:171–184
Coelho A, Costa L, Marques MDM, Fonseca I, Lemos MA, Lemos F (2010) Using simultaneous DSC/TG to analyze the kinetics of polyethylene degradation—catalytic cracking using HY and HZSM-5 zeolites. React Kinet Mech Cat 99:5–15
Osman M, Satti SM, Luqman A, Hassan F, Shah Z, Shah AA (2017) Degradation of polyester polyurethane by Aspergillus sp. strain S45 isolated from soil. J Polym Environ 26:301–310
Shivakumar S, Jagadish SJ, Zatakia H, Dutta J (2011) Purification, characterization and kinetic studies of a novel poly (β) hydroxybutyrate (PHB) depolymerase PhaZ Pen from Penicillium citrinum S2. Appl Biochem Biotechnol 164:1225–1236
Elbanna K, Lutke-Eversloh T, Jendrossek D, Luftmann H, Steinbuchel A (2004) Studies on the biodegradability of polythioester copolymers and homopolymers by polyhydroxyalkanoate (PHA)-degrading bacteria and PHA depolymerases. Arch Microbiol 182:212–225
Akbar S, Hasan F, Nadhman A, Khan S, Shah AA (2013) Production and purification of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) degrading enzyme from Streptomyces sp. AF-111. J Polym Environ 21:1109–1116
Abusham RA, Zaliha NR, Rahman RA, Saleha AB, Basri M (2009) Optimization of physical factors affecting the production of thermo-stable organic solvent-tolerant protease from a newly isolated halo tolerant Bacillus subtilis strain Rand. Microb Cell Fact 8:20
Jablonski S, Krasowska A (2013) Characterisation of alkaline lipase from an arctic yeast strain Rhodosporidium babjevae BD19. Eur Sci J 9(21):17–34
Mao H, Jiang H, Su T, Wang Z (2013) Purification and characterization of two extracellular polyhydroxyalkanoate depolymerases from Pseudomonas mendocina. Biotechnol Lett 35:1919–1924
Gowda USV, Shivakumar S (2015) Poly(-β-hydroxybutyrate) (PHB) depolymerase PHAZ(Pen) from Penicillium expansum: purification, characterization and kinetic studies. 3 Biotech 5(6):901–909
Çolak A, Sisik D, Saglam N, Guner S, Canaksci S, Belduz AO (2005) Characterization of a thermoalkalophilic esterase from a novel thermophilic bacterium, Anoxybacillus gonensis G2. Bioresour Technol 96:625–631
Kim D, Rhee Y (2003) Biodegradation of microbial and synthetic polyesters by fungi. Appl Microbiol Biotechnol 61:300–308
Author information
Authors and Affiliations
Corresponding author
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Satti, S.M., Shah, Z., Luqman, A. et al. Biodegradation of Poly(3-hydroxybutyrate) and Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Newly Isolated Penicillium oxalicum SS2 in Soil Microcosms and Partial Characterization of Extracellular Depolymerase. Curr Microbiol 77, 1622–1636 (2020). https://doi.org/10.1007/s00284-020-01968-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00284-020-01968-7