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Pararcticibacter amylolyticus gen. nov., sp. nov., Isolated from a Rotten Hemp Rope, and Reclassification of Pedobacter tournemirensis as Pararcticibacter tournemirensis comb. nov.

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Abstract

A Gram-stain-negative, rod-shaped, non-motile, facultatively anaerobic bacterium, designated FJ4-8T, was isolated from a rotten hemp rope in Chongqing City, PR China. Phylogenetic analysis of 16S rRNA gene sequences indicated that the isolate was closely related to members of the family Sphingobacteriaceae, with the highest similarity to Pedobacter tournemirensis TF5-37.2-LB10T (95.3%) and low similarities to all other species of the genus Pedobacter (90.4–93.9%). Phylogenetic analyses demonstrated that strain FJ4-8T formed a stable subclade with Pedobacter tournemirensis TF5-37.2-LB10T. The clade with these two strains branched adjacent to a clade containing three species of the genus Arcticibacter. MK-7 was detected as the only respiratory quinone. The major fatty acids composed iso-C15:0, iso-C17:0 3-OH and summed feature three. Phosphatidylethanolamine, three aminophospholipids and one unidentified lipid were found as the major polar lipids. The major polyamine was identified as sym-homospermidine. The DNA–DNA hybridization value between strain FJ4-8T and Pedobacter tournemirensis TF5-37.2-LB10T was 42.0 ± 2.5%. Based on its phylogenetic, chemotaxonomic and phenotypic characteristics, the novel strain and TF5-37.2-LB10T were found to be different from members of genera Pedobacter and Arcticibacter. FJ4-8T and TF5-37.2-LB10T represented different species. Therefore, FJ4-8T should be classified as a novel species of a novel genus in the family Sphingobacteriaceae, for which the name Pararcticibacter amylolyticus gen. nov., sp. nov. is proposed. The type strain is FJ4-8T (= KCTC 62640T = CCTCC AB 2018052T). The draft genome sequence is 6290, 449 bp in length, the genomic DNA G+C content was 44.4 mol%. Pedobacter tournemirensis TF5-37.2-LB10T should be transferred to the novel genus as Pararcticibacter tournemirensis comb. nov. (The type strain is TF5-37.2-LB10T (= DSM 23085T = CIP 110085T = MOLA 820T).

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Acknowledgements

We thank Professor Bernhard Schink for the Latin construction of the genetic name and species name. The fatty acids and polar lipids were analyzed by Guang Dong Detection Center of Microbiology, PR China.

Funding

This work was financially supported by the Special Fund for Agro-scientific Research in the Public Interest (Grant No. 201503137).

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Authors and Affiliations

  1. State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People’s Republic of China

    Yu-yuan Cai, Wei-wei Dong, Yuan-liang Hu, Nan Peng, Yun-xiang Liang & Shu-miao Zhao

  2. Hubei Key Laboratory of Edible Wild Plants Conservation and Utilization, College of Life Sciences, Hubei Normal University, Huangshi, 435002, Hubei, People’s Republic of China

    Yuan-liang Hu

  3. Institute of Agricultural Resources and Regional Planning, CAAS, Beijing, 100081, People’s Republic of China

    Xu Jiang & Yi-qing Zhou

  4. Key Laboratory of Development and Application of Rural Renewable Energy (MOA, China), Biogas Institute of Ministry of Agriculture, Chengdu, 610041, Sichuan, People’s Republic of China

    Yan-wei Wang

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  1. Yu-yuan Cai
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Correspondence to Shu-miao Zhao.

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Cai, Yy., Dong, Ww., Hu, Yl. et al. Pararcticibacter amylolyticus gen. nov., sp. nov., Isolated from a Rotten Hemp Rope, and Reclassification of Pedobacter tournemirensis as Pararcticibacter tournemirensis comb. nov.. Curr Microbiol 77, 320–326 (2020). https://doi.org/10.1007/s00284-019-01828-z

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