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Antibiotic Resistance of LACTOBACILLUS Strains

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Abstract

The study provides phenotypic and molecular analyses of the antibiotic resistance in 20 Lactobacillus strains including 11 strains newly isolated from fermented plant material. According to the results of disc diffusion method, 90% of tested lactobacilli demonstrated sensitivity to clindamycin and 95% of strains were susceptible to tetracycline, erythromycin, and rifampicin. Ampicillin and chloramphenicol were found to inhibit all bacteria used in this study. The vast majority of tested strains revealed phenotypic resistance to vancomycin, ciprofloxacin, and aminoglycosides. Most of Lactobacillus strains showed high minimum inhibitory concentrations (MICs) of cefotaxime, ceftriaxone, and cefazolin and therefore were considered resistant to cephalosporins. All the strains exhibited multidrug resistance. The occurrence of resistance genes was associated with phenotypic resistance, with the exception of phenotypically susceptible strains that contained genes for tetracycline (tetK, tetL) and erythromycin (ermB, mefA) resistance. The vanX gene for vancomycin resistance was among the most frequently identified among the lactobacilli (75% of strains), but the occurrence of the parC gene for ciprofloxacin resistance was sporadic (20% of strains). Our results mainly evidence the intrinsic nature of the resistance to aminoglycosides in lactobacilli, though genes for enzymatic modification of streptomycin aadA and aadE were found in 20% of tested strains. The occurrence of extended spectrum beta-lactamases (ESBL) was unknown in Lactobacillus, but our results revealed the blaTEM gene in 80% of strains, whereas blaSHV and blaOXA-1 genes were less frequent (20% and 15% of strains, respectively).

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Acknowledgements

This work was supported by the program of competitive growth of Kazan Federal University, RFBR Grants No 18–34-00268 and 17–00-00456.

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  1. Department of Microbiology, Institute of Fundamental Medicine and Biology, Kazan Federal University, Kremlevskaya str. 18, Kazan, 420008, Russian Federation

    Elizaveta A. Anisimova & Dina R. Yarullina

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  1. Elizaveta A. Anisimova
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EA designed the experiments, carried out the study, interpreted the data and drafted the manuscript. DY designed the study, supervised all experiments and reviewed the manuscript. All authors read and approved the final manuscript.

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Correspondence to Dina R. Yarullina.

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Anisimova, E.A., Yarullina, D.R. Antibiotic Resistance of LACTOBACILLUS Strains. Curr Microbiol 76, 1407–1416 (2019). https://doi.org/10.1007/s00284-019-01769-7

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