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ArdB Protective Activity for Unmodified λ Phage Against EcoKI Restriction Decreases in UV-Treated Escherichia coli

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Abstract

Anti-restriction proteins ArdB/KlcA specifically inhibit restriction (endonuclease) activity of restriction-modification (RM) type I systems. Molecular mechanisms of ArdB/KlcA-based anti-restriction remain unknown. In this study, we quantitate effects of ArdB on protection of unmodified λ phage DNA from EcoKI restriction. After UV irradiations, which produce significant amounts of unmodified chromosomal DNA in Escherichia coli K12 cells, the protective activity of ArdB decreases. Unlike ArdB, DNA-mimicking protein Ocr retains its ability to protect the unmodified λ phage regardless of UV dose. We hypothesize that the observed decrease in ArdB protective activity in UV-treated cells is due to its binding to unmodified chromosomal DNA, which decreases effective concentrations of free ArdB molecules available for λ phage protection against type I restriction enzymes.

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Funding

Russian Foundation for Basic Research 19-04-00495.

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Authors and Affiliations

  1. Laboratory of Genetics of Bacteria, State Research Institute of Genetics and Selection of Industrial Microorganisms of the National Research Center “Kurchatov Institute”, Moscow, Russia, 115454

    Vladimir P. Balabanov, Olga E. Melkina, Klara S. Pustovoit, Svetlana A. Khrulnova & Gennadii B. Zavilgelsky

  2. Molecular Genetics Lab, Moscow Institute of Physics and Technology, Dolgoprudny, Russia, 141700

    Anna A. Kudryavtseva

  3. Laboratory of Clinical Bacteriology, Mycology, and Antibiotic Treatment, National Research Center for Hematology, Noviy Zykovskiy pr. 4, Moscow, Russia, 125167

    Svetlana A. Khrulnova

Authors
  1. Vladimir P. Balabanov
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  2. Anna A. Kudryavtseva
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  3. Olga E. Melkina
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  4. Klara S. Pustovoit
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  5. Svetlana A. Khrulnova
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  6. Gennadii B. Zavilgelsky
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Correspondence to Anna A. Kudryavtseva.

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Balabanov, V.P., Kudryavtseva, A.A., Melkina, O.E. et al. ArdB Protective Activity for Unmodified λ Phage Against EcoKI Restriction Decreases in UV-Treated Escherichia coli. Curr Microbiol 76, 1374–1378 (2019). https://doi.org/10.1007/s00284-019-01755-z

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  • DOI: https://doi.org/10.1007/s00284-019-01755-z

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