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Regulation of IAA Biosynthesis in Azospirillum brasilense Under Environmental Stress Conditions

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Abstract

Indole-3-acetic acid (IAA) is one of the most important molecules produced by Azospirillum sp., given that it affects plant growth and development. Azospirillum brasilense strains Sp245 and Az39 (pFAJ64) were pre-incubated in MMAB medium plus 100 mg/mL l-tryptophan and treated with or exposed to the following (a) abiotic and (b) biotic stress effectors: (a) 100 mM NaCl or Na2SO4, 4.0% (w/v) PEG6000, 0.5 mM H2O2, 0.1 mM abscisic acid, 0.1 mM 1-aminocyclopropane 1-carboxylic acid, 45 °C or daylight, and (b) 4.0% (v/v) filtered supernatant of Pseudomonas savastanoi (Ps) or Fusarium oxysporum (Fo), 0.1 mM salicylic acid (SA), 0.1 mM methyl jasmonic acid (MeJA), and 0.01% (w/v) chitosan (CH). After 30 and 120 min of incubation, biomass production, cell viability, IAA concentration (µg/mL), and ipdC gene expression were measured. Our results show that IAA production increases with daylight or in the presence of PEG6000, ABA, SA, CH, and Fo. On the contrary, exposure to 45 °C or treatment with H2O2, NaCl, Na2SO4, ACC, MeJA, and Ps decrease IAA biosynthesis. In this report, growth and IAA biosynthesis in A. brasilense under biotic and abiotic stress conditions are discussed from the point of view of their role in bacterial lifestyle and their potential application as bioproducts.

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Acknowledgements

We thank Universidad Nacional de Río Cuarto, Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Fondo Nacional de Ciencia y Tecnología (FONCyT), and Instituto Nacional de Tecnología Agropecuaria (INTA, Argentina). Fabricio Cassán, Veronica Mora, and Susana Rosas are researchers of CONICET at the UNRC. Romina Molina and Diego Rivera are PhD students at the UNRC with grants from CONICET.

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Authors and Affiliations

  1. Universidad Nacional de Río Cuarto, Ruta 36, Km 601, Río Cuarto, Córdoba, Argentina

    Romina Molina, Diego Rivera, Verónica Mora, Gastón López, Susana Rosas & Fabricio Cassán

  2. Katholieke Universiteit Leuven, Leuven, Belgium

    Stijn Spaepen & Jos Vanderleyden

  3. Max Planck Institute for Plant Breeding Research, Plant Microbe Interactions, Cologne, Germany

    Stijn Spaepen

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  1. Romina Molina
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  2. Diego Rivera
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  8. Fabricio Cassán
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Correspondence to Fabricio Cassán.

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Molina, R., Rivera, D., Mora, V. et al. Regulation of IAA Biosynthesis in Azospirillum brasilense Under Environmental Stress Conditions. Curr Microbiol 75, 1408–1418 (2018). https://doi.org/10.1007/s00284-018-1537-6

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