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The Phosphatome of Medicinal and Edible Fungus Wolfiporia cocos

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Abstract

Wolfiporia cocos is an important medicinal and edible fungus that grows in association with pine trees, and its dried sclerotium has been used as a traditional medicine in China for centuries. However, the commercial production of W. cocos sclerotia is currently limited by shortages in pine wood resources. Since protein phosphatases (PPs) play significant roles in growth, signal transduction, development, metabolism, sexual reproduction, cell cycle, and environmental stress responses in fungi, the phosphatome of W. cocos was analyzed in this study by identifying PP genes, studying transcript profiles and assigning PPs to orthologous groups. Fifty-four putative PP genes were putatively identified in W. cocos genome based on homologous sequences searching using BLASTx program against the Saccharomyces cerevisiae, Fusarium graminearum, and Sclerotinia sclerotiorum databases. Based on known and presumed functions of orthologues of these PP genes found in other fungi, the putative roles of these W. cocos PPs in colonization, hyphal growth, sclerotial formation, secondary metabolism, and stress tolerance to environment were discussed in this study. And the level of transcripts from PP genes in the mycelium and sclerotium stages was also analyzed by qRT-PCR. Our study firstly identified and functional discussed the phosphatome in the medicinal and edible fungus W. cocos. The data from our study contribute to a better understanding of PPs potential roles in various cellar processes of W. cocos, and systematically provide comprehensive and novel insights into W. cocos economically important traits that could be extended to other fungi.

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Acknowledgements

We thank the anonymous reviewers for their constructive and helpful comments. This research was supported by Scientific Research Foundation Granted From Wuhan Polytechnic University (2017y12).

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Authors and Affiliations

  1. College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, 430023, People’s Republic of China

    Wenjun Zhu, Shaopeng Zhang, Yonglian Zheng & Ping Chen

  2. Institute for Interdisciplinary Research, Jianghan University, Wuhan, 430056, People’s Republic of China

    Wei Wei

  3. State Key Laboratory of Agrobiotechnology and Ministry of Agriculture Key Laboratory of Plant Pathology, China Agricultural University, Beijing, 100193, People’s Republic of China

    Xiaowen Xu

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  1. Wenjun Zhu
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  2. Wei Wei
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  3. Shaopeng Zhang
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  4. Yonglian Zheng
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  6. Xiaowen Xu
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Contributions

Conceived and designed the experiments: WZ and WW. Performed the experiments: WZ and WW. Analyzed the experiment data: WZ, WW, and SZ. Contributed reagents/materials/analysis tools: WZ, WW, SZ, YZ, and PC. Wrote the paper: WZ and WW. All authors read and approved the final manuscript.

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Correspondence to Wenjun Zhu.

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Zhu, W., Wei, W., Zhang, S. et al. The Phosphatome of Medicinal and Edible Fungus Wolfiporia cocos . Curr Microbiol 75, 124–131 (2018). https://doi.org/10.1007/s00284-017-1356-1

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