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Characterization of Gastric Microbiota in Twins

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Abstract

Contribution of host genetic backgrounds in the development of gastric microbiota has not been clearly defined. This study was aimed to characterize the biodiversity, structure and composition of gastric microbiota among twins. A total of four pairs of twins and eight unrelated individuals were enrolled in the study. Antral biopsies were obtained during endoscopy. The bacterial 16S rRNA gene was amplified and pyrosequenced. Sequences were analyzed for the composition, structure, and α and β diversities of gastric microbiota. Proteobacteria, Firmicutes, Bacteroidetes, Actinobacteria, and Fusobacteria were the most predominant phyla of gastric microbiota. Each individual, twins as well as unrelated individuals, harbored a microbiota of distinct composition. There was no evidence of additional similarity in the richness and evenness of gastric microbiota among co-twins as compared to unrelated individuals. Calculations of θYC and PCoA demonstrated that the structure similarity of gastric microbial community between co-twins did not increase compared to unrelated individuals. In contrast, the structure of microbiota was altered enormously by Helicobacter pylori infection. These results suggest that host genetic backgrounds had little effect in shaping the gastric microbiota. This property of gastric microbiota could facilitate the studies discerning the role of microbiota from genetic grounds in the pathogenesis.

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Authors and Affiliations

  1. Central Laboratories and Department of Gastroenterology, Qingdao Municipal Hospital, Qingdao, 266000, Shandong, China

    Quanjiang Dong, Yongning Xin, Lili Wang, Xinying Meng, Xinjuan Yu, Linlin Lu & Shiying Xuan

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  1. Quanjiang Dong
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  2. Yongning Xin
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Correspondence to Shiying Xuan.

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Dong, Q., Xin, Y., Wang, L. et al. Characterization of Gastric Microbiota in Twins. Curr Microbiol 74, 224–229 (2017). https://doi.org/10.1007/s00284-016-1176-8

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