Skip to main content
SpringerLink
Log in

In Vitro Antiviral Activity of Germacrone Against Porcine Reproductive and Respiratory Syndrome Virus

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

Porcine reproductive and respiratory syndrome (PRRS) is one of the most serious diseases affecting the swine industry worldwide; however, there is no efficient control strategies against PRRSV at present. Therefore, development of new antiviral treatment strategies is urgently needed. As reported, germacrone can efficiently impair influenza virus replication. In this study, we exploited whether germacrone has the potential to inhibit PRRSV infection. Our results showed that the germacrone significantly inhibited replication of PRRSV in vitro and repressed the synthesis of viral RNA and protein. However, it did not block PRRSV binding and entry. Further studies confirmed that germacrone impaired PRRSV replication at an early stage, and inhibited infection of both classic and highly pathogenic type II PRRSV strains. Collectively, our findings imply that the germacrone has the potential to be used as an anti-PRRSV drug.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Allende R, Lewis TL, Lu Z, Rock DL, Kutish GF, Ali A, Doster AR, Osorio FA (1999) North American and European porcine reproductive and respiratory syndrome viruses differ in non-structural protein coding regions. J Gen Virol 80:307–315

    Article  CAS  PubMed  Google Scholar 

  2. Calvert JG, Slade DE, Shields SL, Jolie R, Mannan RM, Ankenbauer RG, Welch SK (2007) CD163 expression confers susceptibility to porcine reproductive and respiratory syndrome viruses. J Virol 81:7371–7379

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Cavanagh D (1997) Nidovirales: a new order comprising Coronaviridae and Arteriviridae. Arch Virol 142:629–633

    CAS  PubMed  Google Scholar 

  4. Cho W, Nam JW, Kang HJ, Windono T, Seo EK, Lee KT (2009) Zedoarondiol isolated from the rhizoma of Curcuma heyneana is involved in the inhibition of iNOS, COX-2 and pro-inflammatory cytokines via the downregulation of NF-kappaB pathway in LPS-stimulated murine macrophages. Int Immunopharmacol 9:1049–1057

    Article  CAS  PubMed  Google Scholar 

  5. Cui J, Fu X, Xie J, Gao M, Hong M, Chen Y, Su S, Li S (2014) Critical role of cellular cholesterol in bovine rotavirus infection. Virol J 11:98

    Article  PubMed  PubMed Central  Google Scholar 

  6. Cui J, Xie J, Gao M, Zhou H, Chen Y, Cui T, Bai X, Wang H, Zhang G (2015) Inhibitory effects of lithium chloride on replication of type II porcine reproductive and respiratory syndrome virus in vitro. Antivir Ther 20:565–572

    Article  CAS  PubMed  Google Scholar 

  7. Dang YY, Li XC, Zhang QW, Li SP, Wang YT (2010) Preparative isolation and purification of six volatile compounds from essential oil of Curcuma wenyujin using high-performance centrifugal partition chromatography. J Sep Sci 33:1658–1664

    Article  CAS  PubMed  Google Scholar 

  8. Das PB, Dinh PX, Ansari IH, de Lima M, Osorio FA, Pattnaik AK (2010) The minor envelope glycoproteins GP2a and GP4 of porcine reproductive and respiratory syndrome virus interact with the receptor CD163. J Virol 84:1731–1740

    Article  CAS  PubMed  Google Scholar 

  9. Feng Y, Zhao T, Nguyen T, Inui K, Ma Y, Nguyen TH, Nguyen VC, Liu D, Bui QA, To LT, Wang C, Tian K, Gao GF (2008) Porcine respiratory and reproductive syndrome virus variants, Vietnam and China, 2007. Emerg Infect Dis 14:1774–1776

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Forsberg R (2005) Divergence time of porcine reproductive and respiratory syndrome virus subtypes. Mol Biol Evol 22:2131–2134

    Article  CAS  PubMed  Google Scholar 

  11. Kimman TG, Cornelissen LA, Moormann RJ, Rebel JM, Stockhofe-Zurwieden N (2009) Challenges for porcine reproductive and respiratory syndrome virus (PRRSV) vaccinology. Vaccine 27:3704–3718

    Article  CAS  PubMed  Google Scholar 

  12. Li Y, Wo JM, Liu Q, Li X, Martin RC (2009) Chemoprotective effects of Curcuma aromatica on esophageal carcinogenesis. Ann Surg Oncol 16:515–523

    Article  PubMed  Google Scholar 

  13. Liao Q, Qian Z, Liu R, An L, Chen X (2013) Germacrone inhibits early stages of influenza virus infection. Antiviral Res 100:578–588

    Article  CAS  PubMed  Google Scholar 

  14. Liu Y, Wang W, Fang B, Ma F, Zheng Q, Deng P, Zhao S, Chen M, Yang G, He G (2013) Anti-tumor effect of germacrone on human hepatoma cell lines through inducing G2/M cell cycle arrest and promoting apoptosis. Eur J Pharmacol 698:95–102

    Article  CAS  PubMed  Google Scholar 

  15. Lunney JK, Benfield DA, Rowland RR (2010) Porcine reproductive and respiratory syndrome virus: an update on an emerging and re-emerging viral disease of swine. Virus Res 154:1–6

    Article  CAS  PubMed  Google Scholar 

  16. Ni J, Yang S, Bounlom D, Yu X, Zhou Z, Song J, Khamphouth V, Vatthana T, Tian K (2012) Emergence and pathogenicity of highly pathogenic Porcine reproductive and respiratory syndrome virus in Vientiane, Lao People’s Democratic Republic. J Vet Diagn Invest 24:349–354

    Article  PubMed  Google Scholar 

  17. Shi M, Holmes EC, Brar MS, Leung FC (2013) Recombination is associated with an outbreak of novel highly pathogenic porcine reproductive and respiratory syndrome viruses in China. J Virol 87:10904–10907

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Snijder EJ, Meulenberg JJ (1998) The molecular biology of arteriviruses. J Gen Virol 79:961–979

    Article  CAS  PubMed  Google Scholar 

  19. Tian K, Yu X, Zhao T, Feng Y, Cao Z, Wang C, Hu Y, Chen X, Hu D, Tian X, Liu D, Zhang S, Deng X, Ding Y, Yang L, Zhang Y, Xiao H, Qiao M, Wang B, Hou L, Wang X, Yang X, Kang L, Sun M, Jin P, Wang S, Kitamura Y, Yan J, Gao GF (2007) Emergence of fatal PRRSV variants: unparalleled outbreaks of atypical PRRS in China and molecular dissection of the unique hallmark. PLoS ONE 2:e526

    Article  PubMed  PubMed Central  Google Scholar 

  20. Xiao X, Zhao Y, Yuan H, Xia W, Zhao J, Wang X (2002) Study on the effect of Rhizoma Curcuma Longa on gastrin receptor. Zhong Yao Cai 25:184–185

    PubMed  Google Scholar 

  21. Xie J, Cui T, Cui J, Chen Y, Zhang M, Zhou P, Deng S, Su S, Zhang G (2014) Epidemiological and evolutionary characteristics of the PRRSV in Southern China from 2010 to 2013. Microb Pathog 75:7–15

    Article  PubMed  Google Scholar 

  22. Zhang M, Cao Z, Xie J, Zhu W, Zhou P, Gu H, Sun L, Su S, Zhang G (2013) Mutagenesis analysis of porcine reproductive and respiratory syndrome virus nonstructural protein 7. Virus Genes 47:467–477

    Article  CAS  PubMed  Google Scholar 

  23. Zhong Z, Chen X, Tan W, Xu Z, Zhou K, Wu T, Cui L, Wang Y (2011) Germacrone inhibits the proliferation of breast cancer cell lines by inducing cell cycle arrest and promoting apoptosis. Eur J Pharmacol 667:50–55

    Article  CAS  PubMed  Google Scholar 

  24. Zhong Z, Chen X, Zhou K, Wu T, Cui L, Wang Y (2010) Rhizoma curcuma exerts synthetic effect of anti-breast cancer through suppressing cell proliferation, metastasis and angiogenesis in vitro. Int J Mol Med 261:S39

    Google Scholar 

Download references

Acknowledgments

This project was supported by the Public Welfare Special Funds for Agriculture Scientific Research (grant number 201203039), the Modern Agro-industry Technology Research System (grant number CARS-36) and the National Natural Science Foundation of China (grant number 31272564).

Author information

Author notes

    Authors and Affiliations

    1. College of Veterinary and National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou, China

      Jiaping Feng, Xiaolei Bai, Tiantian Cui, Han Zhou, Yao Chen, Jiexiong Xie, Qingwei Shi, Heng Wang & Guihong Zhang

    2. Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou, China

      Jiaping Feng, Xiaolei Bai, Tiantian Cui, Han Zhou, Yao Chen, Jiexiong Xie, Qingwei Shi, Heng Wang & Guihong Zhang

    Authors
    1. Jiaping Feng
      View author publications

      You can also search for this author in PubMed Google Scholar

    2. Xiaolei Bai
      View author publications

      You can also search for this author in PubMed Google Scholar

    3. Tiantian Cui
      View author publications

      You can also search for this author in PubMed Google Scholar

    4. Han Zhou
      View author publications

      You can also search for this author in PubMed Google Scholar

    5. Yao Chen
      View author publications

      You can also search for this author in PubMed Google Scholar

    6. Jiexiong Xie
      View author publications

      You can also search for this author in PubMed Google Scholar

    7. Qingwei Shi
      View author publications

      You can also search for this author in PubMed Google Scholar

    8. Heng Wang
      View author publications

      You can also search for this author in PubMed Google Scholar

    9. Guihong Zhang
      View author publications

      You can also search for this author in PubMed Google Scholar

    Corresponding author

    Correspondence to Guihong Zhang.

    Additional information

    Jiaping Feng, Xiaolei Bai and Tiantian Cui contributed equally to this work.

    Rights and permissions

    Reprints and permissions

    About this article

    Check for updates. Verify currency and authenticity via CrossMark

    Cite this article

    Feng, J., Bai, X., Cui, T. et al. In Vitro Antiviral Activity of Germacrone Against Porcine Reproductive and Respiratory Syndrome Virus. Curr Microbiol 73, 317–323 (2016). https://doi.org/10.1007/s00284-016-1042-8

    Download citation

    • Received:

    • Accepted:

    • Published:

    • Issue Date:

    • DOI: https://doi.org/10.1007/s00284-016-1042-8

    Keywords

    Search

    Navigation