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Identification of a Hemagglutinin from Gallibacterium anatis

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Abstract

Gallibacterium anatis has the ability to hemagglutinate rabbit erythrocytes; however, no bacterial component has yet been associated with this function. In the present work, a protein of approximately 65 kDa with hemagglutinating activity for glutaraldehyde-fixed chicken erythrocytes was purified by ion interchange chromatography from G. anatis F149T secreted proteins. The protein was recognized by a rabbit polyclonal serum against a hemagglutinin from Avibacterium paragallinarum. The 65 kDa purified protein presented identity with a G. anatis filamentous hemagglutinin by mass spectrometric analysis. As well, the bacterial surface of G. anatis was labeled by immune gold assays using a polyclonal serum against the 65-kDa protein. A similar protein was recognized in four other G. anatis strains by immunoblots using the same antiserum. The protein binds sheep or pig biotinylated fibrinogen, suggesting an interaction with basement membrane eukaryotic cells components, and the protein is present in G. anatis biofilms. Overall, the results suggest that the 65 kDa hemagglutinin is a common antigen and a potential virulence factor in G. anatis.

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Acknowledgments

This work was supported by Grants from UNAM-DGAPA-PAPIIT IN222313, and FESI-DIP-PAPCA-2014-8. We thank Jose Molina and Lourdes Rojas from the Electron Microscopy Laboratory of CINVESTAV-IPN for their technical assistance.

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Authors and Affiliations

  1. Carrera de Biología, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Av. de los Barrios # 1, Los Reyes Iztacala, 54090, Tlalnepantla, Estado de México, Mexico

    J. F. Montes-García, S. Vaca & E. Negrete-Abascal

  2. Centro de Investigaciones en Ciencias Microbiológicas, Instituto de ciencias, BUAP, Apdo. Postal 1622, 72560, Puebla, Mexico

    C. Vazquez-Cruz

  3. Centro de Investigación y Estudios Avanzados en Salud Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, 50200, Toluca, Mexico

    E. Soriano-Vargas

  4. CENID-Microbiología, Carr. Mexico-Toluca Km15.5, Cuajimalpa, 05110, Mexico, DF, Mexico

    F. Aguilar-Romero

  5. Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, Queensland, 4067, Australia

    P. J. Blackall

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  1. J. F. Montes-García
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  2. S. Vaca
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  3. C. Vazquez-Cruz
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  4. E. Soriano-Vargas
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  5. F. Aguilar-Romero
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  7. E. Negrete-Abascal
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Correspondence to S. Vaca.

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Montes-García, J.F., Vaca, S., Vazquez-Cruz, C. et al. Identification of a Hemagglutinin from Gallibacterium anatis . Curr Microbiol 72, 450–456 (2016). https://doi.org/10.1007/s00284-015-0969-5

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  • DOI: https://doi.org/10.1007/s00284-015-0969-5

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