Abstract
Trichoderma harzianum is an important commercial biocontrol fungal agent. The temperature has been shown to be an important parameter and strain-specific to the mycelia growth of fungi, but less report makes the known of the mechanisms in T. harzianum. In our study, a 6-h treatment of heat increased the thiobarbituric acid reactive substances (TBARS) and nitric oxide (NO) concentration in mycelia to 212 and 230 % the level of the control, respectively. The exogenous NO donor sodium nitroprusside (150 μM) reduced the TBARS concentration to 53 % of that under heat stress (HS). At the same time, the NO-specific scavenger at 250 μM, 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-1-oxyl-3-oxide, prevented the exogenous NO-relieved TBARS accumulation under HS. The increased NO concentration under HS was reduced 41 % by the NO synthase (NOS) inhibitor L-NG-nitroarginine methyl ester, but not the nitrate reductase (NR) inhibitor tungstate. Our study exhibited that NO can protect the mycelia of T. harzianum from HS and reduce the oxidative damage by enhancing the activity of NOS and NR.
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Acknowledgments
This work was supported by the Natural Science Foundation of Shandong Province (ZR2014YL002), National High Technology Research and Development Program (“863” Program) of China (2011AA10A205), Special Fund for Agro-Scientific Research in the Public Interest (201303025), Shandong Provincial Merit-Funded Projects for Returned Overseas Chinese Scholars in Science and Technology Research (Lurenshezi 2013-528), the Programs for International S&T Cooperation Program of China (2011DFA30990; 2007DFA30630), Taishan Research Fellowship, National Natural Science Foundation of China (NSFC 31101479), Promotive Research Fund for Excellent Young and Middle-Aged Scientists of Shandong Province (BS2010NY024), and Shandong Science and Technology Development Project (2010GNC10947).
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Yang Yu and Zijun Yang are contributed equally to this study.
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Yu, Y., Yang, Z., Guo, K. et al. Oxidative Damage Induced by Heat Stress Could be Relieved by Nitric Oxide in Trichoderma harzianum LTR-2. Curr Microbiol 70, 618–622 (2015). https://doi.org/10.1007/s00284-014-0764-8
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DOI: https://doi.org/10.1007/s00284-014-0764-8