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Biofilm Formation, Host-Cell Adherence, and Virulence Genes Regulation of Streptococcus suis in Response to Autoinducer-2 Signaling

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Abstract

Autoinducer-2 (AI-2) is a universal signal molecule mediating intra- and interspecies communication among bacteria. AI-2 is a byproduct of the LuxS enzyme during the catabolism of S-adenosylhomocysteine and plays critical roles in regulating various behaviors of bacteria. In our previous study, the function of LuxS in AI-2 production was verified in Streptococcus suis (SS). Decreased levels of SS biofilm formation and host-cell adherence as well as the inability to produce AI-2 were observed in SS having a luxS mutant gene. In this study, exogenous addition of a low concentration of AI-2 synthesized in vitro was found to promote biofilm formation and host-cell adherence. However, higher concentrations of AI-2 inhibited SS biofilm formation and host-cell adherence. Real-time PCR results showed that the mRNA level of virulence factors of SS biofilm, gdh, cps2, sly, and mrp increased and ef, fbps, and gapdh decreased with increasing AI-2 concentrations. These findings demonstrated that AI-2 supplemented exogenously acted as a concentration-dependent signaling molecule to regulate SS biofilm formation, host-cell adherence, and transcription levels of many virulence genes.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (31201910), the National Basic Research Program of China (2012CB518804), the Science and Technology Research Foundation of Henan Province Educational Committee (13A230261, 14A230003), Foundation for University Key Teacher by the Ministry of Education of Henan Province (2013GGJS-068), Major project of Nanjing Science and Technology Committee (201201026), Cooperative innovation fund of production-college-research of Jiangsu Province (BY2011114), the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions.

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Authors and Affiliations

  1. College of Animal Science and Technology, Henan University of Science and Technology, Luoyang, 471003, China

    Yang Wang & Xiangchao Cheng

  2. Department of Life Science, Luoyang Normal University, Luoyang, 471022, China

    Li Yi

  3. OIE Reference Laboratory for Swine Streptococcosis, Nanjing, 210095, China

    Yang Wang, Li Yi, Zhicheng Zhang, Hongjie Fan & Chengping Lu

  4. Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, 210095, China

    Yang Wang, Li Yi, Zhicheng Zhang, Hongjie Fan & Chengping Lu

  5. Department of Animal Science and Technology, Jinling Institution of Technology, Nanjing, 210038, China

    Zhicheng Zhang

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  1. Yang Wang
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  2. Li Yi
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  3. Zhicheng Zhang
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  4. Hongjie Fan
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  5. Xiangchao Cheng
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Correspondence to Xiangchao Cheng or Chengping Lu.

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Yang Wang and Li Yi have contributed equally to this study.

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Wang, Y., Yi, L., Zhang, Z. et al. Biofilm Formation, Host-Cell Adherence, and Virulence Genes Regulation of Streptococcus suis in Response to Autoinducer-2 Signaling. Curr Microbiol 68, 575–580 (2014). https://doi.org/10.1007/s00284-013-0509-0

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  • DOI: https://doi.org/10.1007/s00284-013-0509-0

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