Abstract
Although intestinal flora are crucial in maintaining immune homeostasis of the intestine, the role of intestinal flora in immune responses at other mucosal surfaces remains less clear. Here, we show that intestinal flora composition critically regulates the toll-like receptor 7 (TLR7) signaling pathway following respiratory influenza virus infection. TLR7 ligands rescued the immune impairment in antibiotic-treated mice. Intact microbiota provided signals leading to the expression of mRNA for TLR7, MyD88, IRAK4, TRAF6, and NF-κB at steady state. Significant changes in the composition of culturable commensal bacteria reduced the expression levels of components of the TLR7 signaling pathway. Our results reveal the importance of intestinal flora in regulating immunity in the respiratory mucosa through the upregulation of the TLR7 signaling pathway for the proper activation of inflammasomes.
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Acknowledgments
This project was supported by the National Natural Science Foundation of China (No. 30973693), (No. 81273616) and the National Major Scientific and National Science and Technology Major projects of the Twelfth Five-year Plan (No. 2013ZX10003001).
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Sha Wu and Zhen-You Jiang contributed equally to this work.
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Wu, S., Jiang, ZY., Sun, YF. et al. Microbiota Regulates the TLR7 Signaling Pathway Against Respiratory Tract Influenza A Virus Infection. Curr Microbiol 67, 414–422 (2013). https://doi.org/10.1007/s00284-013-0380-z
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DOI: https://doi.org/10.1007/s00284-013-0380-z