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Comparative Analysis of Microbial Profiles in Cow Rumen Fed with Different Dietary Fiber by Tagged 16S rRNA Gene Pyrosequencing

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Abstract

The ruminal microbiome of cattle plays an important role not only in animal health and productivity but also in food safety and environment. Microbial profiles of rumen fluid obtained from dairy cows fed on three different fiber/starch diet compositions were characterized. Tagged 16S rRNA gene pyrosequencing and statistical analysis revealed that the dominant ruminal bacteria shared by all three sample groups belonged to phyla Bacteroidetes, Firmicutes, and Proteobacteria. However, the relative abundance of these bacterial groups was markedly affected by diet composition. In animals fed with a high fiber diet, the fibrolytic and cellulolytic bacteria Lachnospiraceae, Ruminococcaceae, and Fibrobacteraceae were found in highest abundance compared with animals fed other diets with lower fiber content. The polysaccharide-degrading Prevotellaceae and Flavobacteriaceae bacteria were most abundant in the rumen of cows fed on diet with the highest starch content. These data highlight the ruminal microbiome’s ability to adapt to feed composition and also provide a basis for the development of feed formulation systems designed to improve livestock productivity.

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Acknowledgments

We thank Genomic Research Laboratory for assistance with pyrosequencing analysis. This study was funded by the Grant P-10-10848 from the National Center for Genetic Engineering and Biotechnology (BIOTEC; Pathumthani, Thailand). Manuscript proofreading by Dr. Philip James Shaw is appreciated.

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Authors and Affiliations

  1. Enzyme Technology Laboratory, Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), 113 Thailand Science Park, Paholyothin Road, Khlong Luang, Pathumthani, 12120, Thailand

    Honglada Thoetkiattikul, Wuttichai Mhuantong, Thanaporn Laothanachareon, Lily Eurwilaichitr & Verawat Champreda

  2. Genomic Research Laboratory, Genome Institute, National Center for Genetic Engineering and Biotechnology, Thailand Science Park, Khlong Luang, Pathumthani, 12120, Thailand

    Sithichoke Tangphatsornruang

  3. Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand

    Virote Pattarajinda

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  1. Honglada Thoetkiattikul
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Correspondence to Honglada Thoetkiattikul.

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Thoetkiattikul, H., Mhuantong, W., Laothanachareon, T. et al. Comparative Analysis of Microbial Profiles in Cow Rumen Fed with Different Dietary Fiber by Tagged 16S rRNA Gene Pyrosequencing. Curr Microbiol 67, 130–137 (2013). https://doi.org/10.1007/s00284-013-0336-3

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