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Roseibacterium beibuensis sp. nov., a Novel Member of Roseobacter Clade Isolated from Beibu Gulf in the South China Sea

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Abstract

A novel aerobic, bacteriochlorophyll-containing bacteria strain JLT1202rT was isolated from Beibu Gulf in the South China Sea. Cells were gram-negative, non-motile, and short-ovoid to rod-shaped with two narrower poles. Strain JLT1202rT formed circular, opaque, wine-red colonies, and grew optimally at 3–4 % NaCl, pH 7.5–8.0 and 28–30 °C. The strain was catalase, oxidase, ONPG, gelatin, and Voges–Proskauer test positive. In vivo absorption spectrum of bacteriochlorophyll a presented two peaks at 800 and 877 nm. The predominant cellular fatty acid was C18:1 ω7c and significant amounts of C16:0, C18:0, C10:0 3-OH, C16:0 2-OH, and 11-methyl C18:1 ω7c were present. Strain JLT1202rT contained Q-10 as the major respiratory quinone and the genomic DNA G+C content was 76.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences of various species with validly published names showed that strain JLT1202rT fell within the genus Roseibacterium, family Rhodobacteraceae, sharing the highest similarity with Roseibacterium elongatum OCh 323T (97.9 % similarity), followed by Dinoroseobacter shibae DFL 12T (95.4 % similarity). The phylogenetic distance of pufM genes between strain JLT1202rT and R. elongatum OCh 323T was 9.4 %, suggesting that strain JLT1202rT was distinct from the only strain of the genus Roseibacterium. Based on the variabilities of phylogenetic and phenotypic characteristics, strain JLT1202rT stands for a novel species of the genus Roseibacterium and the name R. beibuensis sp. nov. is proposed with JLT1202rT as the type strain (=JCM 18015T = CGMCC 1.10994T).

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Acknowledgments

The authors would like to thank Professor Xiaojun Yan, Marine Biotechnology Laboratory, Ningbo University for his valuable help. This study was supported by the 973 Program (2011CB808800), the NSFC Project (91028001), the SOA Project (201105021) and the 863 Program (2012AA092003).

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Authors and Affiliations

  1. State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, 361005, People’s Republic China

    Yujiao Mao, Jingjing Wei, Qiang Zheng, Na Xiao, Qipei Li, Yingnan Fu, Yanan Wang & Nianzhi Jiao

  2. Key Laboratory of Microbial Engineering at the Institute of Biology, Henan Academy of Sciences, Zhengzhou, 450008, People’s Republic China

    Yanan Wang

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  1. Yujiao Mao
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Correspondence to Nianzhi Jiao.

Additional information

The GenBank accession number for the 16S rRNA gene sequence of strain JLT1202rT is JN247667. The pufLM gene sequences of strain JLT1202rT and R. elongatum OCh 323T has been deposited under GenBank accession numbers JQ694098 and JQ694099.

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Mao, Y., Wei, J., Zheng, Q. et al. Roseibacterium beibuensis sp. nov., a Novel Member of Roseobacter Clade Isolated from Beibu Gulf in the South China Sea. Curr Microbiol 65, 568–574 (2012). https://doi.org/10.1007/s00284-012-0192-6

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