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A New Methodology for Differentiation and Typing of Closely Related Salmonella enterica serovar Heidelberg Isolates

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Abstract

This study describe the use of a combination of two recently proposed typing approaches, multiple amplification of prophage locus typing (MAPLT) and multiple-locus variable-number tandem-repeat analysis (MLVA) for subdividing within Salmonella enterica serovar Heidelberg (S. Heidelberg). The combined typing method was compared with pulsed-field gel electrophoresis (PFGE) by Simpson’s index of diversity (DI). PFGE was shown to have a DI = 0.84 and was poor at differentiation of the predominant PT1 (Phage Type 1) phenotype. In comparison, the combined MAPLT/MLVA method comprising 3 MLVA and 9 MAPLT primer pairs provided a higher differentiating ability DI = 0.92. More importantly, the combined methodology was found to be superior in the differentiation of the predominant PT1 isolates. In conclusion, this study demonstrated the potential of the rapid and simple amalgamated MAPLT/MLVA approach in determining transmission of isolates of clonal phage type groups from various environmental sources to humans.

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Acknowledgments

We thank Ms. Dianne Davos, Ms. Helen Hocking and the staff of the Australian Salmonella Reference Centre, Adelaide for providing typed isolates for this study. We also thank the National Health and Medical Research Council for their financial support for this work.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. School of Molecular and Biomedical Science, University of Adelaide, North Terrace, Adelaide, SA, 5005, Australia

    Chun-Chun Young & Michael W. Heuzenroeder

  2. Public Health Unit, Department of Microbiology and Infectious Diseases, SA Pathology (at Women’s and Children’s Hospital), 72 King William Road, North Adelaide, SA, 5006, Australia

    Chun-Chun Young, Ian L. Ross & Michael W. Heuzenroeder

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  1. Chun-Chun Young
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  2. Ian L. Ross
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  3. Michael W. Heuzenroeder
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Correspondence to Michael W. Heuzenroeder.

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Young, CC., Ross, I.L. & Heuzenroeder, M.W. A New Methodology for Differentiation and Typing of Closely Related Salmonella enterica serovar Heidelberg Isolates. Curr Microbiol 65, 481–487 (2012). https://doi.org/10.1007/s00284-012-0179-3

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  • DOI: https://doi.org/10.1007/s00284-012-0179-3

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