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Exogenous Calcium Improves Viability of Biocontrol Yeasts Under Heat Stress by Reducing ROS Accumulation and Oxidative Damage of Cellular Protein

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Abstract

In this article, we investigated the effect of exogenous calcium on improving viability of Debaryomyces hansenii and Pichia membranaefaciens under heat stress, and evaluated the role of calcium in reducing oxidant damage of proteins in the yeast cells. The results indicated that high concentration of exogenous calcium in culture medium was beneficial for enhancing the tolerance of the biocontrol yeasts to heat stress. The possible mechanism of calcium improving the viability of yeasts was attributed to enhancement of antioxidant enzyme activities, decrease in ROS accumulation and reduction of oxidative damage of intracellular protein in yeast cells under heat stress. D. hansenii is more sensitive to calcium as compared to P. membranaefaciens. Our results suggest that application of exogenous calcium combined with biocontrol yeasts is a practical approach for the control of postharvest disease in fruit.

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Acknowledgments

Work in Tian Laboratory was supported by grants from the National Natural Science Foundation of China (31030051; 31172005) and the National High Technology Research (863) Program of China (2012AA101010).

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Authors and Affiliations

  1. Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Nanxincun 20, Xiangshan, Beijing, 100093, People’s Republic of China

    Bang An, Boqiang Li, Guozheng Qin & Shiping Tian

  2. Graduate School of Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China

    Bang An

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  1. Bang An
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  2. Boqiang Li
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  3. Guozheng Qin
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  4. Shiping Tian
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Correspondence to Shiping Tian.

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An, B., Li, B., Qin, G. et al. Exogenous Calcium Improves Viability of Biocontrol Yeasts Under Heat Stress by Reducing ROS Accumulation and Oxidative Damage of Cellular Protein. Curr Microbiol 65, 122–127 (2012). https://doi.org/10.1007/s00284-012-0133-4

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  • DOI: https://doi.org/10.1007/s00284-012-0133-4

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