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Diversity of Genetic Lineages Among CTX-M-15 and CTX-M-14 Producing Escherichia coli Strains in a Tunisian Hospital

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Abstract

Fourteen broad-spectrum-cephalosporin-resistant Escherichia coli isolates were recovered between June and December 2007 in a Tunisian hospital. Genes encoding extended-spectrum-beta-lactamases (ESBL) and other resistance genes were characterized by PCR and sequencing. The following ESBL genes were identified: bla CTX-M-15 (12 isolates), bla CTX-M-14a (one isolate), and bla CTX-M-14b (one isolate). The bla OXA-1 gene was detected in 13 bla CTX-M-producing strains and a bla TEM-1 gene in 6 of them. The ISEcp1 sequence was found upstream of bla CTX-M genes in 8 of 14 strains, and orf477 or IS903 downstream of this gene in 13 strains. Nine of the strains carried class 1 integrons and five different gene cassette arrangements were detected, dfrA17–aadA5 being the most common. One of the strains (bla CTX-M-14a-positive) harbored three class 1 integrons, and one of them was non-previously described containing as gene cassettes new variants of aac(6′)-Ib and cmlA1 genes and it was linked to the bla CTX-M-14a gene flanked by a truncated ISEcp1 sequence (included in GenBank with accession number JF701188). CTX-M-15-producing strains were ascribed to phylogroup B2 (six isolates) and D (six isolates). Multilocus-sequence-typing revealed ten different sequence-types (STs) among ESBL-positive E. coli strains with prevalence of ST405 (four strains of phylogroup D) and ST131 types (two strains of phylogroup B2 and serogroup O25b). A high clonal diversity was also observed among studied strains by pulsed-field-gel-electrophoresis (11 unrelated profiles). CTX-M-15 is an emergent mechanism of resistance in the studied hospital and the world-disseminated 0:25b-ST131-B2 and ST405-D clones have been identified among CTX-M-15-producing isolates.

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Acknowledgments

This study has been possible, thank to an Integrated Action financed by the Spanish Agency of International Collaboration (AECID) from the Ministerio de Asuntos Exteriores of Spain and from the Tunisian Ministry of Higher Education and Scientific Research. V. Estepa has a predoctoral fellowship from the University of La Rioja (Spain) and S. Somalo has a fellowship (project SAF2009-08570 from the Ministerio de Ciencia e Innovación of Spain and FEDER).

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Authors and Affiliations

  1. Faculté des Sciences de Tunis, Laboratoire Microorganismes et Biomolécules Actives, Université Tunis-El Manar, 2092, Tunis, Tunisia

    Karim Ben Slama, Rym Ben Sallem, Ahlem Jouini, Salwa Rachid & Abdellatif Boudabous

  2. Laboratoire de Microbiologie, Polyclinique Ettaoufik, Tunis, Tunisia

    Laurence Moussa

  3. Área de Microbiología Molecular, Centro de Investigación Biomédica de La Rioja, Logroño, Spain

    Yolanda Sáenz & Carmen Torres

  4. Área de Bioquímica y Biología Molecular, Universidad de La Rioja, Madre de Dios, 51, 26006, Logroño, Spain

    Vanesa Estepa, Sergio Somalo & Carmen Torres

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  1. Karim Ben Slama
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  9. Abdellatif Boudabous
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  10. Carmen Torres
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Correspondence to Carmen Torres.

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Ben Slama, K., Ben Sallem, R., Jouini, A. et al. Diversity of Genetic Lineages Among CTX-M-15 and CTX-M-14 Producing Escherichia coli Strains in a Tunisian Hospital. Curr Microbiol 62, 1794–1801 (2011). https://doi.org/10.1007/s00284-011-9930-4

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  • DOI: https://doi.org/10.1007/s00284-011-9930-4

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