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Modulatory Effects of Lactobacillus salivarius on Intestinal Mucosal Immunity of Piglets

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Abstract

Recent studies have demonstrated that lactobacilli or their cell components can improve certain immune function in animals. The aim of this study is to investigate the effects of porcine lactobacilli on the intestinal mucosal immunity of piglets. Neonatal piglets were used as a model and were orally administrated with Lactobacillus salivarius B1 isolated from the duodenal mucosa of a healthy piglet. The feces of the piglets were collected on days 7, 14, and 21 for intestinal microflora analysis. On day 28, the piglets were sacrificed, and their intestinal mucosa samples were immediately collected to investigate the changes in intestinal morphological and immunocompetent cells. Finally, the expression of cytokines and TLRs was detected in the different intestinal segments. The results indicate that L. salivarius B1 can partially ameliorate the microflora of the feces and increase the number of intestinal immunocompetent cells, as the intraepithelial lymphocyte (P < 0.05), and the IgA-producing cells (P < 0.01) in the lactobacilli-treated group were all increased compared with those in the control group. Enhanced expression of the cytokine IL-6 gene was also observed in the ileum (P < 0.05). Moreover, L. salivarius B1 can also upregulate the expression of TLR2 in the intestinal tract at the gene and protein levels (P < 0.05). The results demonstrate that L. salivarius B1 is beneficial for the maturation of the intestinal mucosal immune system and elicited local immunomodulatory activities. In addition, the modulatory effects of L. salivarius B1 on mucosal immunity mainly depend on its extracellular components.

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Acknowledgments

This study was supported by grants (30871858) and (30800791) from the National Science Grant of China and a grant (BE200830155) from the Support Program of Jiangsu province, as well as helps by and Liuhe Breeding Base, Jiangsu Province Academy of Agricultural Sciences.

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Authors and Affiliations

  1. College of Veterinary Medicine, Nanjing Agricultural University, 1 Weigang, Jiangsu, Nanjing, 210095, China

    Jinhua Zhang, Jun Deng, Zhisheng Wang, Chuanyan Che, Yun-feng Li & Qian Yang

  2. College of Animal Science and Technology, Jiangxi Agricultural University, Jiangxi, Nanchang, China

    Jinhua Zhang

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  1. Jinhua Zhang
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  2. Jun Deng
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  3. Zhisheng Wang
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Correspondence to Qian Yang.

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Zhang, J., Deng, J., Wang, Z. et al. Modulatory Effects of Lactobacillus salivarius on Intestinal Mucosal Immunity of Piglets. Curr Microbiol 62, 1623–1631 (2011). https://doi.org/10.1007/s00284-011-9906-4

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  • DOI: https://doi.org/10.1007/s00284-011-9906-4

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