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Curing the Plasmid pXO2 from Bacillus anthracis A16 Using Plasmid Incompatibility

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Abstract

Plasmid incompatibility, which has no effect on other plasmids or chromosomal genes, can be used to cure a target plasmid. In this report, we successfully cured the plasmid pXO2 from Bacillus anthracis A16 with a newly constructed, incompatible plasmid pKSV7-oriIV and obtained a new pXO2-cured strain, designated A16PI2. This is the first time that a plasmid was cured from the B. anthracis wild-type strain A16 utilizing this principle, which could be considered as an efficacious method to cure large plasmids.

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Acknowledgments

This work was supported by the State Major Science and Technology Special Projects of China (No. 2008ZX1004-008) and the National Natural Science Foundation of China (No. 30670104).

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Authors and Affiliations

  1. School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Binhu District, Wuxi, 214122, Jiangsu, China

    Huagui Wang, Xiangru Liao & Hengliang Wang

  2. State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Biotechnology, 20 Dongdajie Street, Fengtai District, Beijing, 100071, People’s Republic of China

    Huagui Wang, Xiankai Liu, Erling Feng, Li Zhu, Dongshu Wang & Hengliang Wang

Authors
  1. Huagui Wang
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  2. Xiankai Liu
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  3. Erling Feng
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  4. Li Zhu
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  5. Dongshu Wang
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  6. Xiangru Liao
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  7. Hengliang Wang
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Corresponding authors

Correspondence to Xiangru Liao or Hengliang Wang.

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Huagui Wang and Xiankai Liu contributed equally to this work.

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Wang, H., Liu, X., Feng, E. et al. Curing the Plasmid pXO2 from Bacillus anthracis A16 Using Plasmid Incompatibility. Curr Microbiol 62, 703–709 (2011). https://doi.org/10.1007/s00284-010-9767-2

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  • DOI: https://doi.org/10.1007/s00284-010-9767-2

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