Abstract
Association between bacteria Photorhabdus and their nematode hosts Heterorhabditis represents one of the emerging models in symbiosis studies. In this study, we isolated the bacterial symbionts of the nematode Heterorhabditis georgiana. Using gyrB sequences for phylogenetic analysis, these strains were shown to be part of the species of Photorhbdus luminescens but with clear separation from currently recognized subspecies. Physiological properties and DNA–DNA hybridization profiles also supported the phylogenetic relationship of these strains. Therefore, a new subspecies, Photorhabdus luminescens subsp. kleinii subsp. nov., is proposed with the type strain KMD37T (=DSM 23513 =ATCC =NRRL B-59419).
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Acknowledgments
This study was supported by a grant from the Ohio Agricultural Research and Development Center of The Ohio State University, Wooster, Ohio. The authors thank Drs. Byron J. Adams and Michael D. Klein for providing nematode strains.
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An erratum to this article can be found at http://dx.doi.org/10.1007/s00284-011-9907-3
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An, R., Grewal, P.S. Photorhabdus luminescens subsp. kleinii subsp. nov. (Enterobacteriales: Enterobacteriaceae). Curr Microbiol 62, 539–543 (2011). https://doi.org/10.1007/s00284-010-9741-z
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DOI: https://doi.org/10.1007/s00284-010-9741-z