Skip to main content
SpringerLink
Log in

Oxidative Damage Involves in the Inhibitory Effect of Nitric Oxide on Spore Germination of Penicillium expansum

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

The effects of nitric oxide (NO) on spore germination of Penicillium expansum were investigated and a possible mechanism was evaluated. The results indicated that NO released by sodium nitroprusside (SNP) significantly suppressed fungal growth. With the use of an oxidant sensitive probe and Western blot analysis, an increased level of intracellular reactive oxygen species (ROS) and enhanced carbonylation damage were detected in spores of P. expansum under NO stress. Exogenous superoxide dismutase (SOD) and ascorbic acid (Vc) could increase the resistance of the spore to the inhibitory effect of NO. The activities of SOD and catalase (CAT), as well as ATP content in spores under NO stress were also lower than those in the control. We suggest that NO in high concentration induces the generation of ROS which subsequently causes severe oxidative damage to proteins crucial to the process of spore germination of P. expansum.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Beltrán B, Mathur A, Duchen MR et al (2000) The effect of nitric oxide on cell respiration: a key to understanding its role in cell survival or death. Proc Natl Acad Sci USA 97:14602–14617

    Article  PubMed  Google Scholar 

  2. Brown GC (1995) Reversible binding and inhibition of catalase by nitric oxide. Eur J Biochem 232:188–191

    Article  CAS  PubMed  Google Scholar 

  3. Carreras MC, Franco MC, Peralta JG et al (2004) Nitric oxide, complex I, and the modulation of mitochondrial reactive species in biology and disease. Mol Aspects Med 25:125–139

    Article  CAS  PubMed  Google Scholar 

  4. Carreras MC, Poderoso JJ (2008) Mitochondrial nitric oxide in the signaling of cell integrated responses. Am J Physiol Cell Physiol 292:1569–1580

    Article  Google Scholar 

  5. Chen CB, Dickman MB (2005) Proline suppresses apoptosis in the fungal pathogen Colletotrichum trifolii. Proc Natl Acad Sci USA 102:3459–3462

    Article  CAS  PubMed  Google Scholar 

  6. Fang FC (1997) Mechanism of nitric oxide-related antimicrobial activity. J Clin Invest 99:2818–2825

    Article  CAS  PubMed  Google Scholar 

  7. Giulivi C, Kato K, Cooper CE (2006) Nitric oxide regulation of mitochondrial oxygen consumption I: cellular physiology. Am J Physiol Cell Physiol 291:1225–1231

    Article  Google Scholar 

  8. Lazar EE, Wills RBH, Ho BT et al (2008) Antifungal effect of gaseous nitric oxide on mycelium growth, sporulation and spore germination of the postharvest horticulture pathogens, Aspergillus niger, Monilinia fructicola and Penicillium italicum. Lett Appl Microbiol 46:688–692

    Article  CAS  PubMed  Google Scholar 

  9. Marek P, Annamalai T, Venkitanarayanan K (2003) Detection of Penicillium expansum by polymerase chain reaction. Int J Food Microbiol 89:139–144

    Article  CAS  PubMed  Google Scholar 

  10. Mur LAJ, Carver TLW, Prats E (2006) NO way to live; the various role of nitric oxide in plant-pathogen interactions. J Exp Bot 57:489–502

    Article  CAS  PubMed  Google Scholar 

  11. Nisoli E, Carruba MO (2006) Nirtic oxide and mitochondrial biogenesis. J Cell Sci 119:2855–2862

    Article  CAS  PubMed  Google Scholar 

  12. Qin GZ, Tian SP (2005) Enhancement of biocontrol activity of Cryptococcus laurentii by silicon and possible mechanisms involved. Phytopathology 1:69–75

    Article  Google Scholar 

  13. Qin GZ, Tian SP, Chan ZL et al (2007) Crucial role of antioxidant proteins and hydrolytic enzymes in pathogenicity of Penicillium expansum. Mol Cell Proteomics 14:425–438

    Google Scholar 

  14. Stewart VC, Sharpe MA, Clark JB et al (2000) Astrocyte-derived nitric oxide cause both reversible and irreversible damage to the neuronal mitochondrial respiratory chain. J Neurochem 75:694–699

    Article  CAS  PubMed  Google Scholar 

  15. Thomas DD, Ridnour LA, Isenberg JS et al (2008) The chemical biology of nitric oxide: implications in cellular signaling. Free Radical Bio Med 45:18–31

    Article  CAS  Google Scholar 

  16. Tian SP, Yao HJ, Deng X et al (2007) Characterization and expression of β-1,3-glucanase genes in jujube fruit induced by the microbial biocontrol agent Cryptococcus laurentii. Phytopathology 3:261–268

    Google Scholar 

  17. Wang J, Higgins VJ (2005) Nitric oxide has a regulatory effect on the germination of conidia of Colletotrichum coccodes. Fungal Genet Biol 42:284–292

    Article  CAS  PubMed  Google Scholar 

  18. Wang Y, Tian SP, Xu Y (2005) Effects of high oxygen concentration on pro- and anti-oxidant enzymes in peach fruits during postharvest periods. Food Chem 91:99–104

    Article  CAS  Google Scholar 

  19. Wills RBH, Ku VVV, Leshem YY (2000) Fumigation with nitric oxide to extend the postharvest life of strawberries. Postharvest Biol Technol 19:75–79

    Article  Google Scholar 

  20. Wink DA, Hanbauer I, Grisham MB et al (1996) Chemical biology of nitric oxide: regulation and protective and toxic mechanisms. Curr Top Cell Regul 34:159–187

    Article  CAS  PubMed  Google Scholar 

  21. Wink DA, Mitchell JB (1998) Chemical biology of nitric oxide: insights into regulatory, cytotoxic, and cytoprotective mechanisms of nitric oxide. Free Radic Biol Med 25:434–456

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This study was supported by National Basic Research Program of China (973 Program) Grant (2006CB1019007), the National Natural Science Foundation of China (30972069), and the Ministry of Science and Technology of China (2006BAD22B02).

Author information

Authors and Affiliations

  1. Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Nanxincun 20, Xiangshan, Beijing, 100093, China

    Tongfei Lai, Boqiang Li, Guozheng Qin & Shiping Tian

  2. Graduate School of Chinese Academy of Sciences, Beijing, 100049, China

    Tongfei Lai

Authors
  1. Tongfei Lai
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Boqiang Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Guozheng Qin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Shiping Tian
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Shiping Tian.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lai, T., Li, B., Qin, G. et al. Oxidative Damage Involves in the Inhibitory Effect of Nitric Oxide on Spore Germination of Penicillium expansum . Curr Microbiol 62, 229–234 (2011). https://doi.org/10.1007/s00284-010-9695-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00284-010-9695-1

Keywords

Search

Navigation