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Quorum Sensing in Aeromonas Species Isolated from Patients in Malaysia

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Abstract

Bacterial quorum sensing signal molecules called N-acylhomoserine lactone (AHL) controls the expression of virulence determinants in many Gram-negative bacteria. We determined AHL production in 22 Aeromonas strains isolated from various infected sites from patients (bile, blood, peritoneal fluid, pus, stool and urine). All isolates produced the two principal AHLs, N-butanoylhomoserine lactone (C4-HSL) and N-hexanoyl homoserine lactone (C6-HSL). Ten isolates also produced additional AHLs. This report is the first documentation of Aeromonas sobria producing C6-HSL and two additional AHLs with N-acyl side chain longer than C6. Our data provides a better understanding of the mechanism(s) of this environmental bacterium emerging as a human pathogen.

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References

  1. Anguita J, Rodriguez-Aparicio LB, Naharro G (1993) Purification, gene cloning, amino acid sequence analysis, and expression of an extracellular lipase from an Aeromonas hydrophila human isolate. Appl Environ Microbiol 59:2411–2417

    CAS  PubMed  Google Scholar 

  2. Chan KG (2009) Exochelin production in Mycobacterium neoaurum. Int J Mol Sci 10:345–353

    Article  CAS  PubMed  Google Scholar 

  3. Eberl L (2006) Quorum sensing in the genus Burkholderia. Int J Med Microbiol 296:103–110

    Article  CAS  PubMed  Google Scholar 

  4. Kamlesh J, Kong R, Patole MS et al (2007) LuxRI homologs are universally present in the genus Aeromonas. BMC Microbiol 7:93

    Article  Google Scholar 

  5. Lee WS, Puthucheary SD (2001) Retrospective study of Aeromonas infection in a Malaysian Urban Area: a 10-year experience. Singap Med J 42:57–60

    CAS  Google Scholar 

  6. Leung KY, Stevenson RM (1988) Tn5-induced protease-deficient strains of Aeromonas hydrophila with reduced virulence for fish. Infect Immun 56:2639–2644

    CAS  PubMed  Google Scholar 

  7. Lynch MJ, Swift S, Kirke DF et al (2002) The regulation of biofilm development by quorum sensing in Aeromonas hydrophila. Environ Microbiol 4:18–28

    Article  CAS  PubMed  Google Scholar 

  8. McClean KH, Winson MK, Fish L et al (1997) Quorum sensing and Chromobacterium violaceum: exploitation of violacein production for the detection of N-acylhomoserine lactones. Microbiology 143:3703–3711

    Article  CAS  PubMed  Google Scholar 

  9. Medina-Martíneza MS, Uyttendaele M, Demolder V et al (2006) Influence of food system conditions on N-acyl homoserine lactones production by Aeromonas spp. Int J Food Microbiol 112:244–252

    Article  Google Scholar 

  10. Merino S, Rubires X, Knøchel S et al (1995) Emerging pathogens: Aeromonas spp. Int J Food Microbiol 28:157–168

    Article  CAS  PubMed  Google Scholar 

  11. Namdari H (1991) Aeromonas species: pathogens of aquatic inhabitants with a human host range. Clin Microbiol Newslett 15:113–116

    Article  Google Scholar 

  12. Noonan B, Trust TJ (1997) The synthesis, secretion and role in virulence of the paracrystalline surface protein layers of Aeromonas salmonicida and A. hydrophila. FEMS Microbiol Lett 154:1–7

    Article  CAS  PubMed  Google Scholar 

  13. Pemberton JM, Kidd SP, Schmidt R (1997) Secreted enzymes of Aeromonas. FEMS Microbiol Lett 152:1–10

    Article  CAS  PubMed  Google Scholar 

  14. Smith RS, Iglewski BH (2003) P. aeruginosa quorum-sensing systems and virulence. Curr Opin Microbiol 6:56–60

    Article  CAS  PubMed  Google Scholar 

  15. Swift S, Karlyshev AV, Fish L et al (1997) Quorum sensing in Aeromonas hydrophila and Aeromonas salmonicida: identification of the luxRI homologues AhyRI and AsaRI and their cognate N-acylhomoserine lactone signal molecules. J Bacteriol 179:5271–5281

    CAS  PubMed  Google Scholar 

  16. Swift S, Lynch MJ, Fish L et al (1999) Quorum sensing-dependent regulation and blockade of exoprotease production in Aeromonas hydrophila. Infect Immun 67:5192–5199

    CAS  PubMed  Google Scholar 

  17. Thornley JP, Shaw JG, Gryllos IA et al (1997) Virulence properties of clinically significant Aeromonas species: evidence for pathogenicity. Rev Med Microbiol 8:61–72

    Google Scholar 

  18. Vadivelu J, Puthucheary SD, Parasakthi N (1991) Exotoxin profiles of clinical isolates of Aeromonas hydrophila. J Med Microbiol 35:363–367

    Article  Google Scholar 

  19. Vadivelu J, Puthucheary SD, Phipps M et al (1995) Possible virulence factors involved in bacteraemia caused by Aeromonas hydrophila. J Med Microbiol 42:171–174

    Article  CAS  PubMed  Google Scholar 

  20. Venturi V, Friscina A, Bertani I et al (2004) Quorum sensing in Burkholderia cepacia complex. Res Microbiol 155:238–244

    Article  CAS  PubMed  Google Scholar 

  21. Whitehead NA, Barnard AM, Slater H et al (2001) Quorum-sensing in Gram-negative bacteria. FEMS Microbiol Rev 25:365–404

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

This work was supported by the Malaysia Ministry of Science, Technology and Innovation under the Science Fund (02-01-03-SF0494) to KGC; and two grants from the University of Malaya namely PJP (Vot F) Research Grant (FS260/2008C) to CSW, and Research University Grant (RG003/09BIO) to KGC, which are all gratefully acknowledged.

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Authors and Affiliations

  1. Institute of Biological Sciences (Division of Genetics and Molecular Biology), Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia

    Kok-Gan Chan, Xin-Yue Chan, Wai-Fong Yin, Cheng-Siang Wong & Wah-Seng See Too

  2. Department of Medical Microbiology, University of Malaya, 50603, Kuala Lumpur, Malaysia

    Savithri D. Puthucheary

  3. Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia

    Kek-Heng Chua

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  1. Kok-Gan Chan
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  2. Savithri D. Puthucheary
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  3. Xin-Yue Chan
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  4. Wai-Fong Yin
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  5. Cheng-Siang Wong
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  6. Wah-Seng See Too
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  7. Kek-Heng Chua
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Correspondence to Kok-Gan Chan.

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Chan, KG., Puthucheary, S.D., Chan, XY. et al. Quorum Sensing in Aeromonas Species Isolated from Patients in Malaysia. Curr Microbiol 62, 167–172 (2011). https://doi.org/10.1007/s00284-010-9689-z

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  • DOI: https://doi.org/10.1007/s00284-010-9689-z

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