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Simultaneous Amplification of Two Bacterial Genes: More Reliable Method of Helicobacter pylori Detection in Microbial Rich Dental Plaque Samples

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Abstract

Polymerase Chain reaction (PCR) assay is considered superior to other methods for detection of Helicobacter pylori (H. pylori) in oral cavity; however, it also has limitations when sample under study is microbial rich dental plaque. The type of gene targeted and number of primers used for bacterial detection in dental plaque samples can have a significant effect on the results obtained as there are a number of closely related bacterial species residing in plaque biofilm. Also due to high recombination rate of H. pylori some of the genes might be down regulated or absent. The present study was conducted to determine the frequency of H. pylori colonization of dental plaque by simultaneously amplifying two genes of the bacterium. One hundred dental plaque specimens were collected from dyspeptic patients before their upper gastrointestinal endoscopy and presence of H. pylori was determined through PCR assay using primers targeting two different genes of the bacterium. Eighty-nine of the 100 samples were included in final analysis. With simultaneous amplification of two bacterial genes 51.6% of the dental plaque samples were positive for H. pylori while this prevalence increased to 73% when only one gene amplification was used for bacterial identification. Detection of H. pylori in dental plaque samples is more reliable when two genes of the bacterium are simultaneously amplified as compared to one gene amplification only.

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Authors and Affiliations

  1. Department of Oral Health Sciences, Shaikh Zayed Medical Complex, Lahore, 54600, Pakistan

    Saima Chaudhry & Ayyaz Ali Khan

  2. Molecular Virology Lab, National Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, Pakistan

    Muhammad Idrees

  3. Department of Microbiology and Virology, Shaikh Zayed Medical Complex, Lahore, Pakistan

    Mateen Izhar

  4. Department of Gastroenterology and Hepatology, Shaikh Zayed Medical Complex, Lahore, Pakistan

    Arshad Kamal Butt

Authors
  1. Saima Chaudhry
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  2. Muhammad Idrees
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  3. Mateen Izhar
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  4. Arshad Kamal Butt
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  5. Ayyaz Ali Khan
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Correspondence to Saima Chaudhry.

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Chaudhry, S., Idrees, M., Izhar, M. et al. Simultaneous Amplification of Two Bacterial Genes: More Reliable Method of Helicobacter pylori Detection in Microbial Rich Dental Plaque Samples. Curr Microbiol 62, 78–83 (2011). https://doi.org/10.1007/s00284-010-9662-x

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  • DOI: https://doi.org/10.1007/s00284-010-9662-x

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