Skip to main content
SpringerLink
Log in

Photorhabdus temperata subsp. stackebrandtii subsp. nov. (Enterobacteriales: Enterobacteriaceae)

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

The bacterial symbiont of the entomopathogenic nematode Heterorhabditis bacteriophora strain GPS11 was characterized by 16S rRNA gene sequence and physiological traits. The phylogenetic tree built upon 16S rRNA gene sequences clustered the GPS11 bacterial isolate with Photorhabdus temperata strains which have been previously isolated from Heterorhabditis species. The phylogenetic tree further identified four subgroups in P. temperata, and the relationships among these subgroups were confirmed by gyrase subunit B (gyrB) gene sequence analysis. The subgroup containing the GPS11 bacterial isolate differs from other subgroups in sequences of 16S rRNA and gyrB gene, physiological traits, nematode host species, and geographic origin. Therefore, the subgroup comprising the GPS11 bacterial isolate is proposed here as a new subspecies: Photorhabdus temperata subsp. stackebrandtii subsp. nov. (type strain GPS11). The type strain has been deposited in ATCC and DSMZ collections.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Akhurst RJ, Mourant RG, Baud L, Boemare N (1996) Phenotypic and DNA relatedness between nematode symbionts and clinical strains of the genus Photorhabdus (Enterobacteriaceae). Int J Syst Bacteriol 46:1034–1041

    Article  CAS  PubMed  Google Scholar 

  2. Akhurst RJ, Boemare NE, Janssen PH, Peel MM, Alfredson DA, Beard CE (2004) Taxonomy of Australian clinical isolates of the genus Photorhabdus and proposal of Photorhabdus asymbiotica subsp. asymbiotica subsp. nov. and P. asymbiotica subsp. australis subsp. nov. Int J Syst Evol Microbiol 54:1301–1310

    Article  CAS  PubMed  Google Scholar 

  3. Boemare N (2002) Biology, taxonomy and systematics of Photorhabdus and Xenorhabdus. In: Gaugler R (ed) Entomopathogenic nematology. CABI Publishing, New York, pp 35–56

    Chapter  Google Scholar 

  4. Burnell AM, Stock SP (2000) Heterorhabditis, Steinernema and their bacterial symbionts—lethal pathogens of insects. Nematology 2:31–42

    Article  Google Scholar 

  5. Duchaud E, Rusniok C, Frangeul L, Buchrieser C, Givaudan A, Taourit S, Bocs S, Boursaux-Eude C, Chandler M, Charles JF, Dassa E, Derose R, Derzelle S, Freyssinet G, Gaudriault S, Médigue C, Lanois A, Powell K, Siguier P, Vincent R, Wingate V, Zouine M, Glaser P, Boemare N, Danchin A, Kunst F (2003) The genome sequence of the entomopathogenic bacterium Photorhabdus luminescens. Nat Biotechnol 21:1307–1313

    Article  CAS  PubMed  Google Scholar 

  6. Fischer-Le Saux M, Viallard V, Brunel B, Normand P, Boemare NE (1999) Polyphasic classification of the genus Photorhabdus and proposal of new taxa: P. luminescens subsp. luminescens subsp. nov., P. luminescens subsp. akhurstii subsp. nov., P. luminescens subsp. laumondii subsp. nov., P. temperata sp. nov., P. temperata subsp. temperata subsp. nov. and P. asymbiotica sp. nov. Int J Syst Bacteriol 49:1645–1656

    Article  PubMed  Google Scholar 

  7. Grewal PS, Lewis EE, Gaugler R (1997) Response of infective stage parasites (Nematoda: Steinernematidae) to volatile cues from infected hosts. J Chem Ecol 23:503–515

    Article  CAS  Google Scholar 

  8. Grewal PS, Grewal SK, Malik VS, Klein MG (2002) Differences in susceptibility of introduced and native white grub species to entomopathogenic nematodes from various geographic localities. Biol Control 24:230–237

    Article  Google Scholar 

  9. Hazir S, Kaya HK, Stock SP, Keskun N (2003) Entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) for biological control of soil pests. Turk J Biol 27:181–202

    Google Scholar 

  10. Hazir S, Stackerbandt E, Lang E, Schumann P, Ehlers RU, Keskin N (2004) Two new subspecies of Photorhabdus luminescens, isolated from Heterorhabditis bacteriophora (Nematoda: Heterorhabditidae): Photorhabdus luminescens subsp. kayaii subsp. nov and Photorhabdus luminescens subsp. thracensis subsp. nov. Syst Appl Microbiol 27:36–42

    Article  CAS  PubMed  Google Scholar 

  11. Joyce SA, Watson RJ, Clarke DJ (2006) The regulation of pathogenicity and mutualism in Photorhabdus. Curr Opin Microbiol 9:1–6

    Article  Google Scholar 

  12. Kalita M, Małek W, Kaznowski A (2004) Analysis of genetic relationship of Sarothamnus scoparius microsymbionts and Bradyrhizobium sp. by hybridization in microdilution wells. J Biosci Bioeng 97:158–161

    CAS  PubMed  Google Scholar 

  13. Marokhazi J, Waterfield N, LeGoff G, Feil E, Stabler R, Hinds J, Fodor A, ffrench-Constant RH (2003) Using a DNA microarray to investigate the distribution of insect virulence factors in strains of Photorhabdus bacteria. J Bacteriol 185:4648–4656

    Article  CAS  PubMed  Google Scholar 

  14. Nielsen O, Lubeck PS (2002) Characterization of symbionts of entomopathogenic nematodes by universally primed-PCR (UP-PCR) and UP-PCR product cross-hybridization. FEMS Microbiol Lett 215:63–68

    Article  CAS  PubMed  Google Scholar 

  15. Poinar GO (1990) Biology and taxonomy of Steinernematidae and Heterorhabditidae. In: Gaugler R, Kaya HK (eds) Entomopathogenic nematodes in biological control. CRC press, Boca Raton, pp 23–62

    Google Scholar 

  16. Power DA (1988) Manual of BBL® products and laboratory procedures, 6th edn. Becton Dickinson Microbiology Systems, Cockeysville

    Google Scholar 

  17. Staley J, Krieg NR (1984) Bacterial classification I. Classification of prokaryotic organisms: an overview. In: Krieg NR, Holt JG (eds) Bergey’s manual of systematic bacteriology, vol 1. The Williams & Wilkins Co, Baltimore, pp 1–4

    Google Scholar 

  18. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599

    Article  CAS  PubMed  Google Scholar 

  19. Tarrand JJ, Groschel DH (1982) Rapid, modified oxidase test for oxidase-variable bacterial isolates. J Clin Microbiol 16:772–774

    CAS  PubMed  Google Scholar 

  20. Tóth T, Lakatos T (2008) Photorhabdus temperata subsp. cinerea subsp. nov., isolated from Heterorhabditis nematodes. Int J Syst Evol Microbiol 58:2579–2581

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

This research was supported by an interdisciplinary grant from the Ohio Agricultural Research and Development Center of The Ohio State University, Wooster, Ohio.

Author information

Authors and Affiliations

  1. Department of Entomology, OARDC, The Ohio State University, 1680 Madison Ave., Wooster, OH, 44691, USA

    Ruisheng An & Parwinder S. Grewal

Authors
  1. Ruisheng An
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Parwinder S. Grewal
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Parwinder S. Grewal.

Rights and permissions

Reprints and permissions

About this article

Cite this article

An, R., Grewal, P.S. Photorhabdus temperata subsp. stackebrandtii subsp. nov. (Enterobacteriales: Enterobacteriaceae). Curr Microbiol 61, 291–297 (2010). https://doi.org/10.1007/s00284-010-9610-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00284-010-9610-9

Keywords

Search

Navigation