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Isolation and Partial Characterization of Antifungal Metabolites Produced by Bacillus sp. IBA 33

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Abstract

Antifungal proteins produced by Bacillus sp. IBA 33 were purified by ammonium sulfate precipitation and DEAE-Sephacel column chromatography. The two purified proteins inhibited the growth of Geotrichum candidum, the sour rot disease agent in lemon. The proteins were stable at 20 (3 months), 40, 60 and 100°C (30 min) and remained active after sterilization at 121°C for 15 min. Their hydrophobic nature was proved and when were developed with ninhydrin they did not show any free amino groups. The infrared spectrum showed vibrational modes corresponding to peptide, ester or ketone links and saturated CH links corresponding to long chain fatty acids. UV scan spectroscopy showed tyrosine and or tryptophan amino acids in their composition. The remarkable thermo-resistance of proteins may be a good feature to be used in the development of a new biocontrol method of Geotrichum candidum.

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Authors and Affiliations

  1. Instituto de Biotecnología, Facultad de Bioquímica Química y Farmacia, Universidad Nacional de Tucumán, Ayacucho 465, 4000, Tucumán, Argentina

    María C. Maldonado, Jose Corona, María A. Gordillo & Antonio R. Navarro

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  1. María C. Maldonado
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  2. Jose Corona
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  3. María A. Gordillo
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  4. Antonio R. Navarro
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Correspondence to María C. Maldonado.

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Maldonado, M.C., Corona, J., Gordillo, M.A. et al. Isolation and Partial Characterization of Antifungal Metabolites Produced by Bacillus sp. IBA 33. Curr Microbiol 59, 646–650 (2009). https://doi.org/10.1007/s00284-009-9489-5

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  • DOI: https://doi.org/10.1007/s00284-009-9489-5

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