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Mycoparasitism of Endophytic Fungi Isolated From Reed on Soilborne Phytopathogenic Fungi and Production of Cell Wall-Degrading Enzymes In Vitro

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Abstract

Antagonism of three endophytic fungi isolated from common reed (Phragmites australis) against eight soilborne pathogenic fungi was investigated on potato dextrose agar by light microscopy, scanning electron microscopy, and transmission electron microscopy. Inhibitory zones were not observed. The microscopical studies suggested that the endophytes inhibit growth of soilborne pathogens by means of coiling around hyphae and, after penetration, the degradation of hyphal cytoplasm. Since penetration of hyphae seems to play a major role in parasitism, we studied the production of cell wall degrading enzymes by the three endophytes. Choiromyces aboriginum produced higher activities of β-1,3-glucanases compared to Stachybotrys elegans and Cylindrocarpon sp. For C. aboriginum and S. elegans, colloidal chitin was the best substrate for the induction of β-1,3-glucanases and chitinases, respectively. This result suggests that mycoparasitism by endophytes on soilborne plant pathogens can be explained by their mycoparasitic activity.

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Acknowledgments

This work was supported by the Deutsche Forschungsgemeinschaft (DFG) through the Collaborative Research Center 454 (Littoral Zone of Lake Constance) and the National Natural Science Foundation of China (30710103021, 30571498, and 30872024) as well as Foundation of Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University (200701).

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Authors and Affiliations

  1. Department of Plant Pathology, Shandong Agricultural University, Taian, 271018, People’s Republic of China

    Ronghua Cao, Kexiang Gao, Jianfeng Gao, Yang Dai & Xue Wang

  2. Institute of Life Sciences, Jiangsu University, Zhenjiang, 212013, People’s Republic of China

    Xiaoguang Liu

  3. Department of Biology, University of Constance, 78457, Constance, Germany

    Kurt Mendgen

  4. Key Laboratory of Molecular Biology for Agriculture, Shaanxi Province, Northwest A & F University, Yangling, 712100, People’s Republic of China

    Zhensheng Kang

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  1. Ronghua Cao
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  2. Xiaoguang Liu
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  4. Kurt Mendgen
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Correspondence to Kexiang Gao or Kurt Mendgen.

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Cao, R., Liu, X., Gao, K. et al. Mycoparasitism of Endophytic Fungi Isolated From Reed on Soilborne Phytopathogenic Fungi and Production of Cell Wall-Degrading Enzymes In Vitro. Curr Microbiol 59, 584–592 (2009). https://doi.org/10.1007/s00284-009-9477-9

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