Skip to main content

Advertisement

SpringerLink
Log in

Interference of Cranberry Constituents in Cell–Cell Signaling System of Vibrio harveyi

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

Cranberry juice has long been recognized in folk medicine as a therapeutic agent, mainly in urinary track infections. It acts as an antibiofilm agent against various pathogens. Quorum sensing is process where bacteria communicate with each other via signal molecules known as autoinducers. This process is strongly involved in various bacterial pathological and physiological pathways. Various strains of Vibrio harveyi bacteria were incubated with different concentrations of nondialyzable material of cranberry (NDM) with or without addition of exogenous autoinducer. Bioluminescence regulated by the autoinducers was measured in GENios reader. Effect of NDM alone or NDM supplemented with autoinducer on quorum sensing was determined as change in bioluminescence in each treated sample compared to appropriate control in every strain. Using model of V. harveyi, we found an inhibitory effect of cranberry constituents on bacterial signaling system. This effect was reversible, since exogenous autoinducer was able to recover bioluminescence which was decreased by NDM. We hypothesized that cranberry NDM interacts with V. harveyi quorum sensing by competition with autoinducer for binding to autoinducer sensor.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Adonizio A, Kong KF, Mathee K (2008) Inhibition of quorum sensing-controlled virulence factor production in Pseudomonas aeruginosa by South Florida plant extracts. Antimicrob Agents Chemother 52:198–203

    Article  PubMed  CAS  Google Scholar 

  2. Aharoni R, Bronstheyn M, Jabbour A, Zaks B, Srebnik M, Steinberg D (2008) Oxazaborolidine derivatives inducing autoinducer-2 signal transduction in Vibrio harveyi. Bioorg Med Chem 16:1596–1604

    Article  PubMed  CAS  Google Scholar 

  3. Bassler BL, Wright M, Showalter RE, Silverman MR (1993) Intercellular signalling in Vibrio harveyi: sequence and function of genes regulating expression of luminescence. Mol Microbiol 9:773–786

    Article  PubMed  CAS  Google Scholar 

  4. Bassler BL, Wright M, Silverman MR (1994) Sequence and function of LuxO, a negative regulator of luminescence in Vibrio harveyi. Mol Microbiol 12:403–412

    Article  PubMed  CAS  Google Scholar 

  5. Bassler BL, Greenberg EP, Stevens AM (1997) Cross-species induction of luminescence in the quorum-sensing bacterium Vibrio harveyi. J Bacteriol 179:4043–4045

    PubMed  CAS  Google Scholar 

  6. Bodet C, Piché M, Chandad F, Grenier D (2006) Inhibition of periodontopathogenderived proteolytic enzymes by a high-molecular-weight fraction isolated from cranberry. J Antimicrob Chemother 57:685–690

    Article  PubMed  CAS  Google Scholar 

  7. Bosgelmez-Tinaz G, Ulusoy S, Ugur A, Ceylan O (2007) Inhibition of quorumsensing-regulated behaviors by Scorzonera sandrasica. Curr Microbiol 55:114–118

    Article  PubMed  CAS  Google Scholar 

  8. Burger O, Ofek I, Tabak M, Weiss EI, Sharon N, Neeman I (2000) A high molecular mass constituent of cranberry juice inhibits helicobacter pylori adhesion to human gastric mucus. FEMS Immunol Med Microbiol 29:295–301

    Article  PubMed  CAS  Google Scholar 

  9. Choo JH, Rukayadi Y, Hwang JK (2006) Inhibition of bacterial quorum sensing by vanilla extract. Lett Appl Microbiol 42:637–641

    PubMed  CAS  Google Scholar 

  10. Duarte S, Gregoire S, Singh AP, Vorsa N, Schaich K, Bowen WH, Koo H (2006) Inhibitory effects of cranberry polyphenols on formation and acidogenicity of Streptococcus mutans biofilms. FEMS Microbiol Lett 257:50–56

    Article  PubMed  CAS  Google Scholar 

  11. Feldman M, Weiss EI, Shemesh M, Ofek I, Bachrach G, Rozen R, Steinberg D (2009) Cranberry constituents affect fructosyltransferase expression in Streptococcus mutans. Alternat Ther Health Med 15:32–38

    Google Scholar 

  12. Fux CA, Costerton JW, Stewart PS, Stoodley P (2005) Survival strategies of infectious biofilms. Trends Microbiol 13:34–40

    Article  PubMed  CAS  Google Scholar 

  13. Higgins DA, Pomianek ME, Kraml CM, Taylor RK, Semmelhack MF, Bassler BL (2007) The major Vibrio cholerae autoinducer and its role in virulence factor production. Nature 450:883–886

    Article  PubMed  CAS  Google Scholar 

  14. Hochman N, Houri-Haddad Y, Koblinski J, Wahl L, Roniger M, Bar-Sinai A, Weiss EI, Hochman J (2008) Cranberry juice constituents impair lymphoma growth and augment the generation of antilymphoma antibodies in syngeneic mice. Nutr Cancer 60:511–517

    Article  PubMed  Google Scholar 

  15. Koo H, Nino de Guzman P, Schobel BD, Vacca Smith AV, Bowen WH (2006) Influence of cranberry juice on glucan-mediated processes involved in Streptococcus mutans biofilm development. Caries Res 40:20–27

    Article  PubMed  CAS  Google Scholar 

  16. Labrecque J, Bodet C, Chandad F, Grenier D (2006) Effects of a high-molecular weight cranberry fraction on growth, biofilm formation and adherence of Porphyromonas gingivalis. J Antimicrob Chemother 58:439–443

    Article  PubMed  CAS  Google Scholar 

  17. Leitão DP, Polizello AC, Ito IY, Spadaro AC (2005) Antibacterial screening of anthocyanic and proanthocyanic fractions from cranberry juice. J Med Food 8:36–40

    Article  PubMed  Google Scholar 

  18. Liu Y, Black MA, Caron L, Camesano TA (2006) Role of cranberry juice on molecular-scale surface characteristics and adhesion behavior of Escherichia coli. Biotechnol Bioeng 93:297–305

    Article  PubMed  CAS  Google Scholar 

  19. Steinberg D, Feldman M, Ofek I, Weiss EI (2004) Effect of a high-molecularweight component of cranberry on constituents of dental biofilm. J Antimicrob Chemother 54:86–89

    Article  PubMed  CAS  Google Scholar 

  20. Rasmussen TB, Bjarnsholt T, Skindersoe ME, Hentzer M, Kristoffersen P, Köte M, Nielsen J, Eberl L, Givskov M (2005) Screening for quorum-sensing inhibitors (QSI) by use of a novel genetic system, the QSI selector. J Bacteriol 187:1799–1814

    Article  PubMed  CAS  Google Scholar 

  21. Steinberg D, Feldman M, Ofek I, Weiss EI (2005) Cranberry high molecular weight constituents promote Streptococcus sobrinus desorption from artificial biofilm. Int J Antimicrob Agent 25:247–251

    Article  CAS  Google Scholar 

  22. Weiss EI, Kozlovsky A, Steinberg D, Lev-Dor R, Bar Ness Greenstein R, Feldman M, Sharon N, Ofek I (2004) A high molecular mass cranberry constituent reduces mutans streptococci level in saliva and inhibits in vitro adhesion to hydroxyapatite. FEMS Microbiol Lett 232:89–92

    Article  PubMed  CAS  Google Scholar 

  23. Weiss EI, Houri-Haddad Y, Greenbaum E, Hochman N, Ofek I, Zakay-Rones Z (2005) Cranberry juice constituents affect influenza virus adhesion and infectivity. Antiviral Res 66:9–12

    Article  PubMed  CAS  Google Scholar 

  24. Yamanaka A, Kouchi T, Kasai K, Kato T, Ishihara K, Okuda K (2007) Inhibitory effect of cranberry polyphenol on biofilm formation and cysteine proteases of Porphyromonas gingivalis. J Periodontal Res 42:589–592

    Article  PubMed  CAS  Google Scholar 

  25. Zhang L, Ma J, Pan K, Go VL, Chen J, You WC (2005) Efficacy of cranberry juice on Helicobacter pylori infection: a double-blind, randomized placebo-controlled trial. Helicobacter 10:139–145

    Article  PubMed  Google Scholar 

  26. Zhu H, Sun SJ (2008) Inhibition of bacterial quorum sensing-regulated behaviors by Tremella fuciformis extract. Curr Microbiol 57:418–422

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This study is part of the Ph.D. thesis of M. Feldman. The authors thank the Israeli cranberry consortium for their valuable advices and support.

Author information

Authors and Affiliations

  1. Institute of Dental Sciences, Faculty of Dental Medicine, Hebrew University-Hadassah, P.O. Box 12272, Jerusalem, 91120, Israel

    Mark Feldman & Doron Steinberg

  2. Department of Prosthodontics, Faculty of Dental Medicine, Hebrew University-Hadassah, Jerusalem, Israel

    Mark Feldman & Ervin I. Weiss

  3. Department of Human Microbiology, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel

    Itzhak Ofek

Authors
  1. Mark Feldman
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ervin I. Weiss
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Itzhak Ofek
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Doron Steinberg
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Doron Steinberg.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Feldman, M., Weiss, E.I., Ofek, I. et al. Interference of Cranberry Constituents in Cell–Cell Signaling System of Vibrio harveyi . Curr Microbiol 59, 469–474 (2009). https://doi.org/10.1007/s00284-009-9462-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00284-009-9462-3

Keywords

Search

Navigation