Abstract
We used fluorescent penicillin Bocillin FL for characterization of control methicillin-resistant Staphylococcus aureus (MRSA) strains belonging to one of four heterogenic classes and comparing them with clinical MRSA isolates. Significant differences in percentage of fluorescent cells and reduction of Bocillin FL binding after incubation with methicillin between control strains from classes I and IV were observed, whereas the strains from classes II and III were differed after incubation with methicillin. According to this criteria, 55.8% of the clinical isolates population were similar to the strain of class IV or homogenic resistant, 11.8% was found as I, and 32.3% were categorized as class II or III. However, continuous diversity of measured features was also discussed.
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Alvarez-Barrientos A, Arroyo J, Canton R, Nombela C, Sanchez-Perez M (2000) Application of flow cytometry to clinical microbiology. Clin Microbiol Rev 13:167–195
Barski P, Piechowicz L, Galinski J, Kur J (1996) Rapid assay for detection of methicillin-resistant Staphylococcus aureus using multiplex PCR. Mol Cell Probes 10:471–475
De Lencastre M, Figueiredo AM, Urban C, Rahal J, Tomasz A (1991) Multiple mechanisms of methicillin resistance and improved methods for detection in clinical isolates of Staphylococcus aureus. Antimicrob Agents Chemother 35:632–639
De Lencastre H, Figueiredo AMS, Tomasz A (1993) Genetic control of population structure in heterogeneous strains of methicillin resistant Staphylococcus aureus. Eur J Clin Microbiol Infect Dis 12:13–18
Finan JE, Rosato AE, Dickinson TM, Ko D, Archer GL (2002) Conversion of oxacillin-resistant staphylococci from heterotypic to homotypic resistance expression. Antimicrob Agents Chemother 46:24–30
Hartman BJ, Tomasz A (1986) Expression of methicillin resistance in heterogeneous strains of Staphylococcus aureus. Antimicrob Agents Chemother 29:85–92
Jarzembowski T, Wiśniewska K, Jóźwik A, Bryl E, Witkowski J (2008) Flow cytometry as a rapid test for detection of penicillin resistance directly in bacterial cells in Enterococcus faecalis and Staphylococcus aureus. Curr Microbiol 57:167–169
National Committee for Clinical Laboratory Standards (2002) Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically, 5th edn. Approved standard M7-A5. National Committee for Clinical Laboratory Standards, Wayne, PA
Okuma K, Iwakawa K, Turnidge JD et al (2002) Dissemination of new methicillin-resistant Staphylococcus aureus clones in the community. J Clin Microbiol 40:4289–4294
Zhao G, Meier TI, Kahl SD, Gee KR, Blaszczak LC (1999) Bocillin fl, a sensitive and commercially available reagent for detection of penicillin-binding proteins. Antimicrob Agents Chemother 43:1124–1128
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Jarzembowski, T., Wiśniewska, K., Jóźwik, A. et al. Heterogeneity of Methicillin-Resistant Staphylococcus aureus Strains (MRSA) Characterized by Flow Cytometry. Curr Microbiol 59, 78–80 (2009). https://doi.org/10.1007/s00284-009-9395-x
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DOI: https://doi.org/10.1007/s00284-009-9395-x