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Protein Profile of Nomuraea rileyi Spore Isolated from Infected Silkworm

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Abstract

Nomuraea rileyi (N. rileyi) is the causative agent of the silkworm, Bombyx mori, green muscardine which can cause severe worldwide economical loss in sericulture. Little is known about N. rileyi at the protein level for this entomopathogenic parasite which belongs to the Ascomycota. Here, we employed proteomic-based approach to identify proteins of N. rileyi spores collected from the dead silkworm. In all, 252 proteins were separated by two-dimensional gel electrophoresis (2-DE), and were subjected to mass spectrometry (MS) analysis, 121 proteins have good MS signal, and 24 of them were identified due to unavailability of genomic information from N. rileyi. This data will be helpful in understanding the biochemistry of N. rileyi.

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Acknowledgments

This study was supported by Grant from the National Basic Research Program of China (No. 2005CB121000), the National “863” project of China (2008AA10Z145) and Jiangsu Sci-Tech Support Project—Agriculture (No. BE2008379).

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Authors and Affiliations

  1. Institute of Life Sciences, Jiangsu University, 301# Xuefu Road, Zhenjiang, 212013, Jiangsu Province, People’s Republic of China

    Lvgao Qin, Xiaoyong Liu, Huiqing Chen, Qin Yao, Zhe Yang & Keping Chen

  2. Center for Physics and Chemistry, Jiangsu University, 301# Xuefu Road, Zhenjiang, 212013, Jiangsu Province, People’s Republic of China

    Jun Li

  3. Beijing Entry-Exit Inspection and Quarantine Bureau, No. 6 Tianshuiyuan Street, Chaoyang District, Beijing, 100026, People’s Republic of China

    Lin Wang

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  1. Lvgao Qin
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  2. Xiaoyong Liu
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  3. Jun Li
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  4. Huiqing Chen
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  5. Qin Yao
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  7. Lin Wang
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  8. Keping Chen
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Correspondence to Keping Chen.

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Qin, L., Liu, X., Li, J. et al. Protein Profile of Nomuraea rileyi Spore Isolated from Infected Silkworm. Curr Microbiol 58, 578–585 (2009). https://doi.org/10.1007/s00284-009-9374-2

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  • DOI: https://doi.org/10.1007/s00284-009-9374-2

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