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Characterization of the First Oral Vagococcus Isolate from a Root-Filled Tooth with Periradicular Lesions

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Abstract

Strain Endo-EH was isolated from a root-filled tooth associated with periradicular lesions. After subculturing on Columbia blood agar, phenotypic and genomic characterizations using different biochemical test systems, automated ribotyping, MALDI-TOF mass spectronomy, antibiotic susceptibility testing, and 16S rRNA gene sequence analysis were applied for further analysis. Phenotypic characterization identified this strain as Vagococcus fluvialis. Riboprint pattern analysis and 16S rRNA sequencing clearly separated it from relevant genera such as Enterococcus and Tetragenococcus and also from other Vagococcus species. This taxon is a new entry to the list of more than 200 microbial species detected in infected root canal systems.

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References

  1. Allerberger F, Fritschel SJ (1999) Use of automated ribotyping of Austrian Listeria monocytogenes isolates to support epidemiological typing. J Microbiol Methods 35:237–244

    Article  PubMed  CAS  Google Scholar 

  2. Cheung GS, Ho MW (2001) Microbial flora of root canal-treated teeth associated with asymptomatic periapical radiolucent lesions. Oral Microbiol Immunol 16:332–337

    Article  PubMed  CAS  Google Scholar 

  3. Chun J, Lee J-H, Jung Y, Kim M, Kim S, Kim BK , Lim YW (2007) EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57:2259–2261

    Article  PubMed  CAS  Google Scholar 

  4. Collins MD, Ash C, Farrow JA, Wallbanks S, Williams AM (1989) 16S ribosomal acid sequence analysis of lactococci and related taxa. Description of Vagococcus fluvialis gen. nov., sp. nov. J Appl Bacteriol 67:453–460

    PubMed  CAS  Google Scholar 

  5. Glocker E, Berning M, Gerrits MM, Kusters JG, Kist M (2005) Real-time PCR screening for 16S rRNA mutations associated with resistance to tetracycline in Helicobacter pylori. Antimicrob Agents Chemother 49:3166–3170

    Article  PubMed  CAS  Google Scholar 

  6. Gomes BP, Pinheiro ET, Gade-Neto CR, Sousa EL, Ferraz CC, Zaia AA, Teixeira FB, Souza-Filho FJ (2004) Microbiological examination of infected dental root canals. Oral Microbiol Immunol 19:71–76

    Article  PubMed  CAS  Google Scholar 

  7. Hancock HH 3rd, Sigurdsson A, Trope M, Moiseiwitsch J (2001) Bacteria isolated after unsuccessful endodontic treatment in a North American population. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 91:579–586

    Article  PubMed  Google Scholar 

  8. Huang MB, Baker CN, Banerjee S, Tenover FC (1992). Accuracy of the E test for determining antimicrobial susceptibilities of staphylococci, enterococci, Campylobacter jejuni, and gram-negative bacteria resistant to antimicrobial agents. J Clin Microbiol 30:3243–3248

    PubMed  CAS  Google Scholar 

  9. Lawson PA, Falsen E, Cotta MA, Whitehead TR (2007) Vagococcus elongatus sp. nov., isolated from a swine-manure storage pit. Int J Syst Evol Microbiol 57:751–754

    Article  PubMed  CAS  Google Scholar 

  10. Maidak BL, Cole JR, Lilburn TG, Parker CT Jr Saxman PR, Farris RJ, Garrity GM, Olsen GJ, Schmidt TM, Tiedje JM (2001) The RDP-II (Ribosomal Database Project). Nucleic Acids Res 29:173–174

    Article  PubMed  CAS  Google Scholar 

  11. Molander A, Reit C, Dahlen G, Kvist T (1998) Microbiological status of root-filled teeth with apical periodontitis. Int Endod J 31:1–7

    Article  PubMed  CAS  Google Scholar 

  12. Pot B, Devriese LA, Hommez J, Miry C, Vandemeulebroecke K, Kersters K, Haesebrouck F (1994) Characterization and identification of Vagococcus fluvialis strains isolated from domestic animals. J Appl Bacteriol 77:362–369

    PubMed  CAS  Google Scholar 

  13. Rocas IN, Jung IY, Lee CY, Siqueira JF Jr (2004) Polymerase chain reaction identification of microorganisms in previously root-filled teeth in a South Korean population. J Endod 30:504–508

    Article  PubMed  Google Scholar 

  14. Sander A, Ruess M, Bereswill S, Schuppler M, Steinbrueckner B (1998) Comparison of different DNA fingerprinting techniques for molecular typing of Bartonella henselae isolates. J Clin Microbiol 36:2973–2981

    PubMed  CAS  Google Scholar 

  15. Schirrmeister JF, Liebenow AL, Braun G, Wittmer A, Hellwig E, Al-Ahmad A (2007) Detection and eradication of microorganisms in root-filled teeth associated with periradicular lesions: an in vivo study. J Endod 33:536–540

    Article  PubMed  Google Scholar 

  16. Schumann P (2007) MALDI-TOF mass spectrometry versus riboprinting in the quality control of bacterial strains. In: Stackebrandt E, Wozniczka M, Weihs V, Sikorski J (eds) Proceedings of the 11th International Conference on Culture Collections. Döring Druck, Braunschweig, Germany

    Google Scholar 

  17. Jr Siqueira JF (2002) Endodontic infections: concepts, paradigms, and perspectives. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 94:281–293

    Article  PubMed  Google Scholar 

  18. Syed SA, Loesche WJ (1972) Survival of human dental plaque flora in various transport media. Appl Microbiol 24:638–644

    PubMed  CAS  Google Scholar 

  19. Teixeira LM, Carvalho MG, Merquior VL, Steigerwalt AG, Brenner DJ, Facklam RR (1997) Phenotypic and genotypic characterization of Vagococcus fluvialis, including strains isolated from human sources. J Clin Microbiol 35:2778–2781

    PubMed  CAS  Google Scholar 

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Acknowledgment

The authors thank Annette Wittmer, Institute of Medical Microbiology and Hygiene, Albert Ludwigs University, Freiburg, Germany, for her excellent technical help and Peter Schumann (DSMZ) for the MALDI-TOF analysis.

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Authors and Affiliations

  1. Department of Operative Dentistry and Periodontology, University Clinic and Dental Hospital, University of Freiburg, Freiburg, Germany

    Ali Al-Ahmad, Jörg F. Schirrmeister & Elmar Hellwig

  2. Department of Microbiology and Hygiene, Institute of Medical Microbiology and Hygiene, University of Freiburg, Freiburg, Germany

    Klaus Pelz

  3. DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Braunschweig, Germany

    Rüdiger Pukall

  4. Department of Operative Dentistry and Periodontology, University School and Dental Hospital, Hugstetter Straße 55, 79106, Freiburg, Germany

    Ali Al-Ahmad

Authors
  1. Ali Al-Ahmad
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  2. Klaus Pelz
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  3. Jörg F. Schirrmeister
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  4. Elmar Hellwig
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  5. Rüdiger Pukall
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Correspondence to Ali Al-Ahmad.

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Al-Ahmad, A., Pelz, K., Schirrmeister, J.F. et al. Characterization of the First Oral Vagococcus Isolate from a Root-Filled Tooth with Periradicular Lesions. Curr Microbiol 57, 235–238 (2008). https://doi.org/10.1007/s00284-008-9182-0

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  • DOI: https://doi.org/10.1007/s00284-008-9182-0

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