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The Campylobacter jejuni/coli cjaA (cj0982c) Gene Encodes an N-Glycosylated Lipoprotein Localized in the Inner Membrane

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Abstract

The Campylobacter coli 72Dz/92 cjaA gene (orthologue of cj0982c of C. jejuni NCTC 11168) product is a highly immunogenic, amino acid–binding protein. CjaA was palmitic acid-modified when processed in E. coli. In addition, site-directed mutagenesis of the Cys residue of the LAAC motif of its signal sequence confirmed that CjaA is a lipoprotein when processed in Campylobacter. Localization of the protein appeared to be host dependent. In Campylobacter, CjaA was recovered mainly as an inner-membrane protein, whereas in E. coli most of the protein was present in the periplasmic space. Interestingly, antiserum raised against Campylobacter glycine-extracted material also recognized CjaA produced by Campylobacter and Escherichia coli, indicating that at least part of the protein may be surface exposed. Site-directed mutagenesis of the Asn residues of two putative N-linked glycosylation sites (NIS and NFT) showed that CjaA is glycosylated and that only the first N-X-S/T sequeon serves as a glycan acceptor.

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Acknowledgments

This work was supported by the Ministry of Scientific Research and Information Technology (Grants No. 6 P06K 043 21, P04A 052 21, and 2P04C 015 27). We are grateful to R. Curtiss III for providing the rabbit serum that reacted with the Campylobacter glycine-extracted proteins and to J. M. Bolla for providing the anti-Omp50 serum. PACYC184/pgl was kindly provided by M. Wacker. We thank J. Hansen for critical reading of the manuscript.

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  1. Joanna Życka

    Present address: Department of Microbial Biochemistry, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland

Authors and Affiliations

  1. Department of Bacterial Genetics, Institute of Microbiology, University of Warsaw, Warsaw, Poland

    Agnieszka Wyszyńska, Joanna Życka, Renata Godlewska & Elżbieta K. Jagusztyn-Krynicka

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  1. Agnieszka Wyszyńska
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Correspondence to Agnieszka Wyszyńska.

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Wyszyńska, A., Życka, J., Godlewska, R. et al. The Campylobacter jejuni/coli cjaA (cj0982c) Gene Encodes an N-Glycosylated Lipoprotein Localized in the Inner Membrane. Curr Microbiol 57, 181–188 (2008). https://doi.org/10.1007/s00284-008-9171-3

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