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Variation in the Nature of Organic Acid Secretion and Mineral Phosphate Solubilization by Citrobacter sp. DHRSS in the Presence of Different Sugars

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Abstract

A novel phosphate solubilizing bacterium (PSB) was isolated from the rhizosphere of sugarcane and is capable of utilizing sucrose and rock phosphate as the sole carbon and phosphate source, respectively. This PSB exhibited mineral phosphate solubilizing (MPS) phenotype on sugars such as sucrose and fructose, which are not substrates for enzyme glucose dehydrogenase (GDH), along with GDH substrates, viz., glucose, xylose, and maltose, as carbon sources. PCR amplification of the rRNA gene and sequence analysis identified this bacterium as Citrobacter sp. DHRSS. On sucrose and fructose Citrobacter sp. DHRSS liberated 170 and 100 μM free phosphate from rock phosphate and secreted 49 mM (2.94 g/L) and 35 mM (2.1 g/L) acetic acid, respectively. Growth of Citrobacter sp. DHRSS on sucrose is mediated by an intracellular inducible neutral invertase. Interestingly, in the presence of GDH substrates like glucose and maltose, Citrobacter sp. DHRSS produced approximately 20 mM (4.36 g/L) gluconic acid and phosphate released was 520 and 570 μM, respectively. Citrobacter sp. DHRSS GDH activity was found when grown on GDH and non-GDH substrates, indicating that it is constitutive and could act on a wide range of aldose sugars. This study demonstrates the role of different organic acids in mineral phosphate solubilization by rhizobacteria depending on the nature of the available carbon source.

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References

  1. Ae N, Arihara J, Okada K (1991) Phosphorus response of chick pea and evaluation of phosphorus availability in Indian alfisols and vertisols. In: Johansen C, Lee KK, Sharawat KL (eds) Phosphorus nutrition of grain legumes. ICRISAT, India, pp 33–41

    Google Scholar 

  2. Ames BN (1964) Assay of inorganic phosphate, total phosphate and phosphatases. Methods Enzymol 8:115–118

    Google Scholar 

  3. Bolan NS, Mahimairaja S, Baskaran S (1994) Influence of low-molecular-weight organic acids on the solubilization of phosphates. Biol Fertil Soils 18:311–319

    Article  CAS  Google Scholar 

  4. Cerezine PC, Nahas E, Banzatto DA (1988) Soluble phosphate accumulation by Aspergillus niger from fluorapatite. Appl Microbiol Biotechnol 29:501–505

    Article  CAS  Google Scholar 

  5. Cunningham JE, Kuiack C (1992) Production of citric and oxalic acids and solubilization of calcium phosphate by Penicillium bilaii. Appl Environ Microbiol 58:1451–1458

    PubMed  CAS  Google Scholar 

  6. Goldstein AH, Rogers RD, Mead G (1993) Mining by microbe. Bio/Technol 11:1250–1254

    CAS  Google Scholar 

  7. Goldstein AH (1995) Recent progress in understanding the molecular genetics and biochemistry of calcium phosphate solubilization by gram negative bacteria. Biol Agr Hort 12:185–193

    Google Scholar 

  8. Gyaneshwar P, Naresh Kumar G, Parekh LJ (1998) Effect of buffering on the phosphate solubilizing ability of microorganisms. World J Microbiol Biotechnol 14:669–673

    Article  CAS  Google Scholar 

  9. Gyaneshwar P, Parekh LJ, Archana G, Poole PS, Collins MD, Hutson RA, Naresh Kumar G (1999) Involvement of a phosphate starvation inducible glucose dehydrogenase in soil phosphate solubilization by Enterobacter asburiae. FEMS Microbiol Lett 171:223–229

    Article  CAS  Google Scholar 

  10. Gyaneshwar P, Naresh Kumar G, Parekh LJ, Poole PS (2002) Role of soil microorganisms in improving P nutrition of plants. Plant Soil 245:83–93

    Article  CAS  Google Scholar 

  11. Jaeger III CH, Lindow SE, Miller W, Clark E, Firestone MK (1999) Mapping of sugar and amino acid availability in soil around roots with bacterial sensors of sucrose and tryptophan. Appl Environ Microbiol 65:2685–2690

    PubMed  CAS  Google Scholar 

  12. Kamilova F, Kravchenko LV, Shaposhnikov AI, Azarova T, Makarova N, Lugtenberg B (2006) Organic acids, sugars, and L-tryptophane in exudates of vegetables growing on stonewool and their effects on activities of rhizosphere bacteria. Mol Plant Microbe Interact 19:250–256

    Article  PubMed  CAS  Google Scholar 

  13. Katznelson H, Bose B (1959) Metabolic activity and phosphate dissolving capability of bacterial isolates from wheat roots in the rhizosphere and non rhizosphere soil. Can J Microbiol 5:79–85

    Article  PubMed  CAS  Google Scholar 

  14. Kpomblekou K, Tabatabai M (1994) Effects of organic acids on release of phosphorous from rock phosphate. Soil Sciences 158:442–453

    Article  Google Scholar 

  15. Kucey RMN, Jenzen HH, Leggett ME (1989) Microbially mediated increases in plant available phosphorus. Adv Agron 42:199–228

    Article  CAS  Google Scholar 

  16. Kumar V, Narula N (1999) Solubilization of inorganic phosphates and growth emergence of wheat as affected by Azotobacter chroococcum mutants. Biol Fertil Soils 28:301–305

    Article  CAS  Google Scholar 

  17. Lugtenberg JJ, Kravchenko LV, Simons M (1999) Tomato seed and root exudate sugars: composition, utilisation by Pseudomonas biocontrol strains and role in rhizosphere colonisation. Environ Microbiol 1:439–446

    Article  PubMed  CAS  Google Scholar 

  18. Matsushita K, Ameyama M (1982) D-Glucose-dehydrogenase from Pseudomonas fluorescens, membrane bound. Methods Enzymol 89:149–154

    PubMed  CAS  Google Scholar 

  19. Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426

    Article  CAS  Google Scholar 

  20. Narsian V, Thakkar J, Patel HH (1995) Mineral phosphate solubilization by Aspergillus aculeatus. Ind J Exp Biol 33:91–93

    CAS  Google Scholar 

  21. Odunfa SA, Werner D (1981) Root exudates in relation to growth and nitrogenase activity of Rhizobium japonicum. Z Allg Mikrobiol 21:601–606

    Article  PubMed  CAS  Google Scholar 

  22. Peterson GL (1979) Review of the Folin phenol quantitation method of Lowry, Rosenberg, Farr and Randall. Anal Biochem 100:201

    Article  PubMed  CAS  Google Scholar 

  23. Raul R, Velazquez E, Zurdo-Pineiro JL, Mateos PF, Molina EM (2004) Identification of microorganisms by PCR amplification and sequencing of a universal amplified ribosomal region present in both prokaryotes and eukaryotes. J Microbiol Methods 56:413–426

    Article  CAS  Google Scholar 

  24. Reyes I, Bernier L, Simard RR, Antoun H (1999) Effect of nitrogen source on the solubilization of different inorganic phosphates by an isolate of Penicillium rugulosum and two UV-induced mutants. FEMS Microbiol Ecol 28:281–290

    Article  CAS  Google Scholar 

  25. Reyes I, Bernier L, Simard RR, Tanguay P, Antoun H (1999) Characteristics of phosphate solubilization by an isolate of a tropical Penicillium rugulosum and two UV-induced mutants. FEMS Microbiol Ecol 28:291–295

    Article  CAS  Google Scholar 

  26. Rovira AD (1965) Plant root exudates and their influence upon soil microorganisms. In: Baker KF, Snyder WC (eds) Ecology of soil-borne plant pathogens; prelude to biological control, Murray, London, pp 170–186

    Google Scholar 

  27. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY

    Google Scholar 

  28. Sharma V, Kumar V, Archana G, Naresh Kumar G (2005) Substrate specificity of glucose dehydrogenase (GDH) of Enterobacter asburiae PSI3 and rock phosphate solubilization with GDH substrates as C sources. Can J Microbiol 51:477–482

    Article  PubMed  CAS  Google Scholar 

  29. Theodorou ME, Plaxton WC (1993) Metabolic adaptations of plant respiration to nutritional phosphate deprivation. Plant Physiol 101:339–344

    PubMed  CAS  Google Scholar 

  30. Tsay SS, To KY (1987) Citric acid production using immobilized conidia of Aspergillus niger TMB 2022. Biotechnol Bioeng 29:297–304

    Article  CAS  PubMed  Google Scholar 

  31. Vassilev N, Franco I, Vassileva M, Azcon R (1996) Improved plant growth with rock phosphate solubilized by Aspergillus niger grown on sugar-beet waste. Bioresource Technol 55:237–241

    Article  CAS  Google Scholar 

  32. Vassileva M, Vassilev N, Azcon R (1998) Rock phosphate solubilization by Aspergillus niger on olive cake-based medium and its further application in a soil–plant system. World J Microbiol Biotechnol 14:281–284

    Article  CAS  Google Scholar 

  33. Vance CP (2001) Symbiotic nitrogen fixation and phosphorus acquisition. Plant nutrition in a world of declining renewable resources. Plant Physiol 127:390–397

    Article  PubMed  CAS  Google Scholar 

  34. Wakelin SA, Warren RA, Harvey PR, Ryder MH (2004) Phosphate solubilization by Penicillium spp. closely associated with wheat roots. Biol Fertil Soils 40:36–43

    Article  CAS  Google Scholar 

  35. Yadav KS, Dadarwal KR (1997) Phosphate solubilization and mobilization through soil microorganisms. In: Biotechnological approaches in soil microorganisms for sustainable crop production. Scientific Publishers, Jodhpur, India, pp 293–308

  36. Zheng Z, Yao S, Lin D (2005) Using a kinetic model that considers cell segregation to optimize hEGF expression in fed-batch cultures of recombinant Escherichia coli. Bioprocess Biosyst Eng 27:143–152

    Article  PubMed  CAS  Google Scholar 

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Acknowledgment

Helpful comments by an unknown referee are gratefully acknowledged.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Science, M. S. University of Baroda, Vadodara, 390002, India

    Divya K. Patel & G. Naresh Kumar

  2. Department of Microbiology, Faculty of Science, M. S. University of Baroda, Vadodara, 390002, India

    G. Archana

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  1. Divya K. Patel
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  2. G. Archana
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  3. G. Naresh Kumar
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Correspondence to G. Naresh Kumar.

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Patel, D.K., Archana, G. & Kumar, G.N. Variation in the Nature of Organic Acid Secretion and Mineral Phosphate Solubilization by Citrobacter sp. DHRSS in the Presence of Different Sugars. Curr Microbiol 56, 168–174 (2008). https://doi.org/10.1007/s00284-007-9053-0

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