Abstract
Screening the collection of natural isolates from semi-hard homemade cheese resulted in isolation and characterization of strain Lactobacillus paracasei subsp. paracasei BGSJ2-8. The strain BGSJ2-8 harbors several important phenotypes, such as bacteriocin production, aggregation phenomenon, and production of proteinase. Bacteriocin SJ was purified by three-step chromatography. Mass spectrometry established molecular mass of the active peptide at 5372 Da. The auto-aggregation phenotype of wild-type (WT) strain was mediated by secreted aggregation-promoting factor (protein of molecular mass > 200 kDa), probably acting in cooperation with other cell surface protein(s). Comparative study of WT and its spontaneous nonaggregating derivative revealed that aggregation factor was responsible for the observed differences in the bacteriocin and proteinase activities. Bacteriocin SJ activity and resistance to different stresses were higher in the presence of aggregating factor. In contrast, proteinase activity was stronger in the nonaggregating derivative.
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Acknowledgments
Part of this study was enabled by FEMS Young Scientist Grant awarded to J. L., EcoNET Program No. 08139XA, and Pavle Savic No. 11107QG of the French Ministry of Foreign Affairs. This work was also supported by Ministry of Science, Republic of Serbia (MSRS) Grant No. 143036.
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Lozo, J., Jovcic, B., Kojic, M. et al. Molecular Characterization of a Novel Bacteriocin and an Unusually Large Aggregation Factor of Lactobacillus paracasei subsp. paracasei BGSJ2-8, a Natural Isolate from Homemade Cheese. Curr Microbiol 55, 266–271 (2007). https://doi.org/10.1007/s00284-007-0159-1
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DOI: https://doi.org/10.1007/s00284-007-0159-1