Skip to main content
SpringerLink
Log in

Biological Pretreatment with Two Bacterial Strains for Enzymatic Hydrolysis of Office Paper

  • Published:
Current Microbiology Aims and scope Submit manuscript

Abstract

The cellulose-hydrolyzing strains, Sphingomonas paucimobilis MK1 and Bacillus circulans MK2, were separated from soil and were grown together in a single culture plate. Growth B. circulans MK2 in liquid culture required symbiosis with S. paucimobilis MK1. Biological pretreatment with the combined strain suspension after the liquid culture improved enzymatic hydrolysis of office paper from municipal wastes. Sugar recovery by S. paucimobilis MK1 (51%) was 1.4 times higher than that of the untreated sample (30%) and in the strain combination with B. circulans MK2, recovery was further improved by 2.5 times (75%). The sugar recovery in maximum condition was enhanced up to 94% for office paper. Furthermore, biological pretreatment effects were confirmed for more than 1 day less time. In X-ray diffraction patterns for the crystallinity of cellulose in office paper changed after biological pretreatment, the crystallinity was increased in comparison to that in untreated paper. The mechanism of biological pretreatment effect was explained by the fact that the strain acted as an endoglucanase, which hydrolyzes amorphous areas randomly.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.
Fig. 3.

Similar content being viewed by others

Literature Cited

  1. Sun Y, Cheng J (2002) Hydrolysis of lignocellulosic materials for ethanol production: review. Bioresources Technol 83:1–11

    Article  CAS  Google Scholar 

  2. Hsu TA, Himmel M, Schell D, Farmer J, Berggren M (1996) Design and initial operation of a high-solids, pilot-scale reactor for dilute-acid pretreatment of lingocellulosic biomass. Appl Biochem Biotechnol 57/58:3–18

    Article  CAS  Google Scholar 

  3. Schell DJ, Farmer J, Newman M, McMillan JD (2003) Dilute-sulfuric acid pretreatment of corn stover in pilot-scale reactor. Appl Biochem Biotechol 105–108:69–85

    Article  Google Scholar 

  4. Ander P, Eriksson KE (1977) Selective degradation of wood components by white-rot fungi. Physiol Plant 41:239–248

    Article  CAS  Google Scholar 

  5. Hatakka AI (1983) Pretreatment of wheat straw by white-rot fungi for enzymic saccharification of cellulose. Eur J Appl Microbiol Biotechnol 18:350–357

    Article  CAS  Google Scholar 

  6. Boominathan K, Reddy CA (1992) cAMP-mediated differential regulation of lignin peroxidase and manganese-dependent peroxidase production in the white-rot basidiomycete Phanerochaete chrysosporium. Proc Natl Acad Sci (USA) 89:5586–5590

    Article  CAS  Google Scholar 

  7. Sneath PHA, Mair NS, Sharpe ME, Holt JG (ed.) (1986) Bergey’s manual of systematic bacteriology. Vol. 2. Baltimore, Williams and Wilkins

    Google Scholar 

  8. Huang SY, Chen JC (1988) Ethanol production in simultaneous saccharification and fermentation of cellulose with temperature profiling. J Ferment Technol 66: 509–516

    Article  CAS  Google Scholar 

  9. Gaudet G, Gaillard B (1987) Vesicle formation and cellulose degradation in Bacteroides succinogenes cultures: ultrastructural aspects. Arch Microbiol 148:150–154

    Article  CAS  Google Scholar 

  10. Morris EJ, Cole OJ (1987) Relationship between cellulite activity and adhesion to cellulose in Ruminococcus albus. J Gen Microbiol 133:1023–1032

    CAS  Google Scholar 

  11. Bhikhabhai B, Johansson G, Pettersson G (1984) Cellobiohydrolase from Trichoderma reesei: internal homology and prediction of secondary structure. Int J Peptide Protein Res 25:368–374

    Article  Google Scholar 

  12. Tomme P, Tilbeurgh H, Pettersson G, Damme J, Vandekerckhove J, Knowels J, Teeri T, Claeyssens M (1988) Studies of the cellulolytic system of Trichoderma reesei QM9414. Eur J Biochem 170:575–581

    Article  PubMed  CAS  Google Scholar 

  13. Gilkes NR, Warren RAJ, Miller RCJ, Kilburn DG (1988) Precise excision of the cellulose binding domains from two Cellulomonas fimi cellulases by a homologous protease and the effect on catalysis. J Biol Biochem 263:10401–10407

    CAS  Google Scholar 

  14. Maglione G, Matsushita O, Russel JB, Wilson DB (1992) Properties of a genetically reconstructed Prevotella ruminicola endoglucanase. Appl Environ Microbiol 58:3593–3597

    PubMed  CAS  Google Scholar 

  15. Eriksson KE, Pettersson B (1975) Extracellular enzyme system utilized by the fungus Sporotrichum pullverulentum (Chrysosporium lignorum) for the breakdown of cellulose 1. Separation, purification and physicochemical characterization of five endo-l,4-β-glucanases. Eur J Biochem 51:193–206

    Article  PubMed  CAS  Google Scholar 

  16. Wood TM, McCare SI (1978) The cellulase of Trichoderma koningii. Purification and properties of some endoglucanase components with special reference to their action on cellulose when acting alone or in synergism with the cellobiohydrolase. Biochem J 171:61–72

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Applied Biological Science, Fukuyama University, Sanzou, Gakuenchou 1 banchi, Hiroshima, Fukuyama, 729-0292, Japan

    Masahiro Kurakake, Nozomi Ide & Toshiaki Komaki

Authors
  1. Masahiro Kurakake
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Nozomi Ide
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Toshiaki Komaki
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Masahiro Kurakake.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kurakake, M., Ide, N. & Komaki, T. Biological Pretreatment with Two Bacterial Strains for Enzymatic Hydrolysis of Office Paper. Curr Microbiol 54, 424–428 (2007). https://doi.org/10.1007/s00284-006-0568-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00284-006-0568-6

Keywords

Search

Navigation