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Genetic and Comparative Transcriptome Analysis of Bromodomain Factor 1 in the Salt Stress Response of Saccharomyces cerevisiae

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Abstract

The Saccharomyces cerevisiae BDF1 gene, which encodes a bromodomain-containing transcription factor, was previously isolated by transposon mutugenesis in a screen for salt-sensitive mutants. However, the salt stress response mechanism regulated by bromodomain transcription factor 1 protein (Bdf1p) remains poorly understood. In this report, genetic analysis indicated that the salt sensitivity of the BDF1 deletion mutant was suppressed by increased gene dosage of its homologous gene BDF2. Furthermore, comparative transcriptome analysis revealed that the differences in transcriptional response between the wild type and the bdf1Δ mutant in the presence of salt stress (0.6 mol/L NaCl, 45 min) were mainly related to cell-wall biosynthesis, the mitochondria, and several unknown genes. Our results provided further information about the regulatory mechanism involved in the salt stress response and adds new insight for understanding the biological functional of bromdomain-containing proteins in cellular processes.

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Literature Cited

  1. Bianchi MM, Costanzo G, Chelstowska A, Grabowska D, Mazzoni C, Piccinni E, Cavalli A, Ciceroni F, Rytka J, Slonimski PP, Frontali L, Negri R (2004) The bromodomain-containing protein Bdf1p acts as a phenotypic and transcriptional multicopy suppressor of YAF9 deletion in yeast. Mol Microbiol 53:953–968

    Article  PubMed  CAS  Google Scholar 

  2. Bianchi MM, Ngo S, Vandenbol M, Sartori G, Morlupi A, Ricci C, Stefani S, Morlino GB, Hilger F, Carignani G, Slonimski PP, Frontali L (2001) Large-scale phenotypic analysis reveals identical contributions to cell functions of known and unknown yeast genes. Yeast 18:1397–1412

    Article  PubMed  CAS  Google Scholar 

  3. Butcher RA, Schreiber SL (2003) A small molecule suppressor of FK506 that targets the mitochondria and modulates ionic balance in Saccharomyces cerevisiae. Chem Biol 10:521–531

    Article  PubMed  CAS  Google Scholar 

  4. Caro LH, Tettelin H, Vossen JH, Ram AF, van den Ende H, Klis FM (1997) In silicio identification of glycosyl-phosphatidylinositol-anchored plasma-membrane and cell wall proteins of Saccharomyces cerevisiae. Yeast 13:1477–1489

    Article  PubMed  CAS  Google Scholar 

  5. Gasch AP, Spellman PT, Kao CM, Carmel-Harel O, Eisen MB, Storz G, Botstein D, Brown PO (2000) Genomic expression programs in the response of yeast cells to environmental changes. Mol Biol Cell 11:4241–4257

    PubMed  CAS  Google Scholar 

  6. Causton HC, Ren B, Koh SS, Harbison CT, Kanin E, Jennings EG, Lee TI, True HL, Lander ES, Young RA (2001) Remodeling of yeast genome expression in response to environmental changes. Mol Biol Cell 12:323–337

    PubMed  CAS  Google Scholar 

  7. Chua P,Roeder GS (1995) Bdf1, a yeast chromosomal protein required for sporulation. Mol Cell Biol 15:3685–3696

    PubMed  CAS  Google Scholar 

  8. de Jesus Ferreira MC, Bao X, Laize V, Hohmann S (2001) Transposon mutagenesis reveals novel loci affecting tolerance to salt stress and growth at low temperature. Curr Genet 40:27–39

    Article  PubMed  Google Scholar 

  9. Garcia-Rodriguez LJ, Valle R, Duran A, Roncero C (2005) Cell integrity signaling activation in response to hyperosmotic shock in yeast. FEBS Lett 579:6186–6190

    Article  PubMed  CAS  Google Scholar 

  10. Gerik KJ, Donlin MJ, Soto CE, Banks AM, Banks IR, Maligie MA, Selitrennikoff CP, Lodge JK (2005) Cell wall integrity is dependent on the PKC1 signal transduction pathway in Cryptococcus neoformans. Mol Microbiol 58:393–408

    Article  PubMed  CAS  Google Scholar 

  11. Gietz RD, Schiestl RH, Willems AR, Woods RA (1995) Studies on the transformation of intact yeast cells by the LiAc/SS-DNA/PEG procedure. Yeast 11:355–360

    Article  PubMed  CAS  Google Scholar 

  12. Guo Y, Guo H, Zhang L, Xie H, Zhao X, Wang F, Li Z, Wang Y, Ma S, Tao J, Wang W, Zhou Y, Yang W, Cheng J (2005) Genomic analysis of anti-hepatitis B virus (HBV) activity by small interfering RNA and lamivudine in stable HBV-producing cells. J Virol 79:14392–14403

    Article  PubMed  CAS  Google Scholar 

  13. Haro R, Garciadeblas B, Rodriguez-Navarro A (1991) A novel P-type ATPase from yeast involved in sodium transport. FEBS Lett 291:189–191

    Article  PubMed  CAS  Google Scholar 

  14. Jung US, Levin DE (1999) Genome-wide analysis of gene expression regulated by the yeast cell wall integrity signalling pathway. Mol Microbiol 34:1049–1057

    Article  PubMed  CAS  Google Scholar 

  15. Levin DE (2005) Cell wall integrity signaling in Saccharomyces cerevisiae. Microbiol Mol Biol Rev 69:262–291

    Article  PubMed  CAS  Google Scholar 

  16. Márquez JA, R Serrano (1996) Multiple transduction pathways regulate the sodium-extrusion gene PMR2/ENA1 during salt stress in yeast. FEBS Lett 382:89–92

    Article  PubMed  Google Scholar 

  17. Maruyama T, Farina A, Dey A, Cheong J, Bermudez VP, Tamura T, Sciortino S, Shuman J, Hurwitz J, Ozato K (2002) A Mammalian bromodomain protein, brd4, interacts with replication factor C and inhibits progression to S phase. Mol Cell Biol 22:6509–6520

    Article  PubMed  CAS  Google Scholar 

  18. Matangkasombut O, Buratowski RM, Swilling NW, Buratowski S (2000) Bromodomain factor 1 corresponds to a missing piece of yeast TFIID. Genes Dev 14:951–962

    PubMed  CAS  Google Scholar 

  19. Nehlin JO, Carlberg M, Ronne H (1989) Yeast galactose permease is related to yeast and mammalian glucose transporters. Gene 85:313–319

    Article  PubMed  CAS  Google Scholar 

  20. Posas F, Chambers JR, Heyman JA, Hoeffler JP, de Nadal E , Arino J (2000) The transcriptional response of yeast to saline stress. J Biol Chem 275:17249–17255

    Article  PubMed  CAS  Google Scholar 

  21. Sawa C, Nedea E, Krogan N, Wada T, Handa H, Greenblatt J, Buratowski S (2004) Bromodomain factor 1 (Bdf1) is phosphorylated by protein kinase CK2. Mol Cell Biol 24:4734–4742

    Article  PubMed  CAS  Google Scholar 

  22. Wadskog I, Adler L (2003) Ion homeostasis in Saccharomyces cerevisiae under NaCl stress. In: S Hohmann, P Mager (eds) Topics in current genetics: yeast stress responses, vol.1. New York, Springer-Verlag, pp 201–239

    Google Scholar 

  23. Walfridsson M, Anderlund M, Bao X, Hahn-Hägerdal B (1997) Expression of different levels of enzymes from the Pichia stipitis XYL1 and XYL2 genes in Saccharomyces cerevisiae and its effect on product formation during xylose utilisation. Appl Microbiol Biotechnol 48:218–224

    Article  PubMed  CAS  Google Scholar 

  24. Yang XJ (2004) Lysine acetylation and the bromodomain: a new partnership for signaling. Bioessays 26:1076–1087

    Article  PubMed  CAS  Google Scholar 

  25. Yang YH, Dudoit S, Luu P, Lin DM, Peng V, Ngai J, Speed TP (2002) Normalization for cDNA microarray data: a robust composite method addressing single and multiple slide systematic variation. Nucleic Acids Res 30:e15

    Article  PubMed  Google Scholar 

  26. Yoshimoto H, Saltsman K, Gasch AP, Li HX, Ogawa N, Botstein D, Brown PO, Cyert MS (2002) Genome-wide analysis of gene expression regulated by the calcineurin/Crz1p signaling pathway in Saccharomyces cerevisiae. J Biol Chem 277(34):31079–31088

    Article  PubMed  CAS  Google Scholar 

  27. Zeng L, Zhou MM (2002) Bromodomain: an acetyl-lysine binding domain. FEBS Lett 513:124–128

    Article  PubMed  CAS  Google Scholar 

  28. Zhang L, Zhang Y, Zhou Y, An S, Zhou Y, Cheng J (2002) Response of gene expression in Saccharomyces cerevisiae to amphotericin B and nystatin measured by microarrays. Int J Antimicrob Chemother 20:444–450

    Article  CAS  Google Scholar 

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Acknowledgments

This work was supported by the Natural Science Foundation of China (grant no. 30170021, 30671143 and 30570031). In addition, we are grateful to Dr. Roberta Greenwood for manuscript editing.

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Authors and Affiliations

  1. State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, P.R. China

    Xiangyong Liu, Xiaohua Zhang, Chao Wang, Liangyu Liu & Xiaoming Bao

  2. Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI, 53226, USA

    Ming Lei

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  1. Xiangyong Liu
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  2. Xiaohua Zhang
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  4. Liangyu Liu
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Correspondence to Xiaoming Bao.

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Liu, X., Zhang, X., Wang, C. et al. Genetic and Comparative Transcriptome Analysis of Bromodomain Factor 1 in the Salt Stress Response of Saccharomyces cerevisiae . Curr Microbiol 54, 325–330 (2007). https://doi.org/10.1007/s00284-006-0525-4

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  • DOI: https://doi.org/10.1007/s00284-006-0525-4

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