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Effect of Mycoplasmas on Apoptosis of 32D Cells Is Species-Dependent

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Abstract

We previously showed that mycoplasmal infection effectively prevented apoptosis of infected cells, whereas other researchers have indicated that mycoplasmal infection promoted apoptosis. To understand the mechanism underlying this discrepancy, five different species of mycoplasmas were investigated for their effects on apoptosis of interleukin (IL)-3–dependent 32D cells. Results revealed that Mycoplasma fermentans and M. penetrans effectively supported continuous growth of 32D cells after IL-3 withdrawal. M. fermentans was more potent than M. penetrans. This effect was achieved by way of preventing apoptosis and stimulating cell proliferation. On the contrary, M. hominis and M. salivarium accelerated apoptosis of 32D cells. M. genitalium had no significant effect on apoptosis. The RNase protection assay indicated that the proapoptotic and antiapoptotic mycoplasmas altered the expression of major apoptosis regulatory genes differently. The difference in apoptosis regulatory gene expression induced by different species of mycoplasmas might be accountable for their effects on host cell apoptosis.

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Acknowledgments

We are grateful to Tamara Newsome and Joe F. Rodriguez for their critical reviews of this manuscript. This work was supported by grants (UBUY, UBPA, UBIM, and UBZZ) from American Registry of Pathology/Armed Forces Institute of Pathology.

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  1. Division of Molecular Pathobiology, Department of Infectious and Parasitic Diseases Pathology, American Registry of Pathology, Armed Forces Institute of Pathology, AFIP Building 54, Room 3025, 14th Street and Alaska Avenue NW, Washington, DC, 20306-6000, USA

    Shimin Zhang & Shyh-Ching Lo

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  1. Shimin Zhang
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  2. Shyh-Ching Lo
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Correspondence to Shimin Zhang.

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Zhang, S., Lo, SC. Effect of Mycoplasmas on Apoptosis of 32D Cells Is Species-Dependent. Curr Microbiol 54, 388–395 (2007). https://doi.org/10.1007/s00284-006-0491-x

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  • DOI: https://doi.org/10.1007/s00284-006-0491-x

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