Abstract
Genetic characterization of afsK-av (SAV3816) in Streptomyces avermitilis ATCC 31272 was performed to evaluate the role(s) of this eukaryotic-type serine–threonine protein kinase (STPK) in the regulation of morphologic differentiation and secondary metabolism. The afsK-av::neo mutant (SJW4001) was defective in sporulation, melanogenesis, and avermectin production. These phenotypic defects were complemented by introduction of either the intact afsK-av or the 900-nt catalytic domain region. The catalytic domain restored sporulation and melanogenesis to SJW4001 whereas it partially recovered avermectin production. This study reveals that AfsKav is a pleiotropic regulator and demonstrates in vivo that the C-region of AfsKav is not essential for its regulatory role in S. avermitilis differentiations.
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Acknowledgments
This work was supported by grants from the Korea Science and Engineering Foundation (KOSEF-R01-2003-000-10283-0) and by support from the Ministry of Education’s Brain Korea 21 project. The investigators also appreciate the support from the 21C Frontier Microbial Genomics and Application Center Program, Ministry of Science and Technology (Grant No. MG 05-0303-1-0).
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Rajkarnikar, A., Kwon, HJ., Ryu, YW. et al. Catalytic Domain of AfsKav Modulates Both Secondary Metabolism and Morphologic Differentiation in Streptomyces avermitilis ATCC 31272. Curr Microbiol 53, 204–208 (2006). https://doi.org/10.1007/s00284-006-0062-1
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DOI: https://doi.org/10.1007/s00284-006-0062-1