Abstract
To reveal the genetic diversity of Pasteurella pneumotropica, the 16S rDNA sequence and multiple alignments were performed for 35 strains (from 17 mice, 13 rats, 3 hamsters, 1 rabbit, and 1 guinea pig) identified as P. pneumotropica using a commercial biochemical test kit or PCR test and two reference strains (ATCC 35149 and CNP160). Each strain showed a close similarity with one of the following organisms: P. pneumotropica (M75083), Bisgaard taxon22 (AY172726), Pasteurella sp. MCCM00235 (AF224300), Pasteurellaceae gen. sp. Forsyth A3 (AF224301), and Actinobacillus muris (AF024526) on GenBank, and were divided into six clusters on a phylogenic tree. Two reference strains, P. pneumotropica biotype Jawetz and Heyl, were classified at both ends of the clusters. Our conclusion is that P. pneumotropica should be reclassified because of the very wide genetic diversity that exists.
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Acknowledgments
This study was supported by a grant (No. 14208097) from the Ministry of Education, Culture, Sports, Science and Technology, Japan. The authors thank the Pp working group of Japan (Mr. T. Aiba and Dr. T. Narita, Sankyo Co.; Dr. K. Itoh, The University of Tokyo; Ms. M. Kato, Charles River Japan; Dr. E. Kawamoto, Tokyo Medical University; Mr. S. Kiyokawa, CLEA Japan; Ms. Y. Morichika, Japan SLC; Dr. T. Muraguchi, Kyoto University; Dr. Y .Okajima, Mercian Cleantec) and Dr. Ken-ichi Yagami of Tsukuba University for providing some strains.
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Hayashimoto, N., Takakura, A. & Itoh, T. Genetic Diversity on 16S rDNA Sequence and Phylogenic Tree Analysis in Pasteurella pneumotropica Strains Isolated from Laboratory Animals. Curr Microbiol 51, 239–243 (2005). https://doi.org/10.1007/s00284-005-4541-6
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DOI: https://doi.org/10.1007/s00284-005-4541-6