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Production of a Metal-Binding Exopolysaccharide by Paenibacillus jamilae Using Two-Phase Olive-Mill Waste as Fermentation Substrate

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Abstract

The present study investigated the use of two-phase olive mill waste (TPOMW) as substrate for the production of exopolysaccharide (EPS) by the endospore-forming bacilli Paenibacillus jamilae. This microorganism was able to grow and produce EPS in aqueous extracts of TPOMW as a unique source of carbon. The effects of substrate concentration and the addition of inorganic nutrients were investigated. Maximal polymer yield in 100-ml batch-culture experiments (2 g l−1) was obtained in cultures prepared with an aqueous extract of 20% TPOMW (w/v). An inhibitory effect was observed on growth and EPS production when TPOMW concentration was increased. Nutrient supplementation (nitrate, phosphate, and other inorganic nutrients) did not increase yield. Finally, an adsorption experiment of Pb (II), Cd (II), Cu (II), Zn (II), Co (II), and Ni (II) by EPS is reported. Lead was preferentially complexed by the polymer, with a maximal uptake of 230 mg/g EPS.

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Acknowledgments

This research was supported by a grant from the Ministerio de Educación y Ciencia and funded under Project No. REN 2000-1502. We also thank our college Lia Wrightsmith, for revising the English text.

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Authors and Affiliations

  1. Department of Microbiology, Faculty of Pharmacy, University of Granada, Campus Universitario de Cartuja s/n, Granada, 18071, Spain

    Jose Antonio Morillo, Margarita Aguilera, Alberto Ramos-Cormenzana & Mercedes Monteoliva-Sánchez

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  1. Jose Antonio Morillo
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  2. Margarita Aguilera
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  3. Alberto Ramos-Cormenzana
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  4. Mercedes Monteoliva-Sánchez
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Correspondence to Alberto Ramos-Cormenzana.

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Morillo, J.A., Aguilera, M., Ramos-Cormenzana, A. et al. Production of a Metal-Binding Exopolysaccharide by Paenibacillus jamilae Using Two-Phase Olive-Mill Waste as Fermentation Substrate. Curr Microbiol 53, 189–193 (2006). https://doi.org/10.1007/s00284-005-0438-7

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  • DOI: https://doi.org/10.1007/s00284-005-0438-7

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