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Verification of Degradation of n-Alkanes in Diesel Oil by Pseudomonas aeruginosa Strain WatG in Soil Microcosms

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Abstract

Degradation of n-alkanes in diesel oil by Pseudomonas aeruginosa strain WatG (WatG) was verified in soil microcosms. The total petroleum hydrocarbon (TPH) degradation level in two bioaugmentation samples was 51% and 46% for 1 week in unsterilized and sterilized soil microcosms, respectively. The TPH degradation in the biostimulation was of control level (15%). The TPH degradation in aeration-limited samples was clearly reduced when compared with that in aeration-unlimited ones under both sterilized and unsterilized conditions. Addition of WatG into soil microcosms was accompanied by dirhamnolipid production only in the presence of diesel oil. These findings suggest that degradation of n-alkanes in diesel oil in soil microcosms would be facilitated by bioaugmentation of WatG, with production of dirhamnolipid, and also by participation of biostimulated indigenous soil bacteria.

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Literature Cited

  1. Breslow N (1970) A general Kruskal-Wallis test for comparing K-samples subject to unequal patterns of censorship. Biometrika 57:579–594

    Google Scholar 

  2. Briones AM, Okabe S, Umemiya Y, Ramsing N-B, Reichardt W, Okuyama H (2003) Ammonia-oxidizing bacteria on root biofilms and their possible contribution to N use efficiency of different rice cultivars. Plant Soil 250:335–348

    CAS  Google Scholar 

  3. Christie WW (1982) Lipid analyses, 2nd ed. Oxford, United Kingdom: Pergamon Press

    Google Scholar 

  4. Dean SM, Jin Y, Cha DK, Wilson SV, Radosevich M (2001) Phenanthrene degradation in soils co-inoculated with phenanthrene-degrading and biosurfactant-producing bacteria. J Environ Qual 30:1126–1133

    CAS  PubMed  Google Scholar 

  5. Gallego JLR, Loredo J, Llamas JF., Vázquez F, Sánchez J (2001) Bioremediation of diesel-contaminated soils: Evaluation of potential in situ techniques by study of bacterial degradation. Biodegradation 12:325–335

    Article  CAS  PubMed  Google Scholar 

  6. Hori K, Marsudi S, Unno H (2002) Simultaneous production of polyhydroxyalkanoates and rhamnolipids by Pseudomonas aeruginosa. Biotechnol Bioeng 78:699–707

    Article  CAS  PubMed  Google Scholar 

  7. Leahy JG, Colwell RR (1990) Microbial degradation of hydrocarbons in the environment. Microbiol Rev 54:305–315

    CAS  PubMed  Google Scholar 

  8. Margesin R, Schinner F (2001) Biodegradation and bioremediation of hydrocarbons in extreme environments. Appl Microbiol Biotechnol 56:650–663

    Article  CAS  PubMed  Google Scholar 

  9. Rahman KSM, Rahman TJ, Kourkoutas Y, Petsas I, Marchant R, Banat IM (2003) Enhanced bioremediation of n-alkane in petroleum sludge using bacterial consortium amended with rhamnolipid and micronutrients. Biores Technol 90:159–168

    CAS  Google Scholar 

  10. Sim L, Ward OP, Li Z-Y (1997) Production and characterisation of a biosurfactant isolated from Pseudomonas aeruginosa UW-1. J Ind Microbiol Biotechnol 19:232–238

    CAS  PubMed  Google Scholar 

  11. Trindade PVO, Sobral LG, Rizzo ACL, Leite SGF, Soriano AU (2005) Bioremediation of a weathered and a recently oil-contaminated soils from Brazil: A comparison study. Chemosphere 58:515–522

    Article  CAS  PubMed  Google Scholar 

  12. US EPA (1986) Sonication extraction procedure–Method 3550, 3rd edn. Ohio: US EPA

    Google Scholar 

  13. Wongsa P, Tanaka M, Ueno A, Hasanuzzaman M, Yumoto I, Okuyama H (2004) Isolation and characterization of novel strains of Pseudomonas aeruginosa and Serratia marcescens possessing high efficiency to degrade gasoline, kerosene diesel oil, and lubricating oil. Curr Microbiol 49:415–422

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

This work was partly supported by Northern Advancement Center for Science & Technology and the Sumitomo Foundation.

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Authors and Affiliations

  1. Laboratory of Environmental Molecular Biology, Graduate School of Environmental Earth Science, Hokkaido University, Kita-ku, Sapporo, Hokkaido, 060-0810, Japan

    Akio Ueno & Hidetoshi Okuyama

  2. ROM Co. Ltd, Chuo-ku, Sapporo, Hokkaido, 064-0804, Japan

    Mohammad Hasanuzzaman

  3. Genomic Resources & Environmental Adaptation Research Group, Research Institute of Genome-based Biofactory, National Institute of Advanced Industrial Science and Technology (AIST), Toyohira-ku, Sapporo, Hokkaido, 062-8517, Japan

    Isao Yumoto

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  1. Akio Ueno
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  2. Mohammad Hasanuzzaman
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  3. Isao Yumoto
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  4. Hidetoshi Okuyama
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Correspondence to Hidetoshi Okuyama.

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Ueno, A., Hasanuzzaman, M., Yumoto, I. et al. Verification of Degradation of n-Alkanes in Diesel Oil by Pseudomonas aeruginosa Strain WatG in Soil Microcosms. Curr Microbiol 52, 182–185 (2006). https://doi.org/10.1007/s00284-005-0133-8

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  • DOI: https://doi.org/10.1007/s00284-005-0133-8

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