Abstract
Alternaria tenuissima, the parasitic fungus, was obtained from the pruned upper-cut surfaces of mulberry stems. This fungus contains dark pigment because of the presence of melanin in the cell wall. To obtain less-pigmented cell walls, this fungus was grown under dark condition. When the pigmented and less-pigmented cell walls were chemically analyzed, no differences were observed in amino-acid composition, hexoses, or pentoses. However, in pigmented cell walls, higher contents of melanin (2.6%) were found than in less-pigmented cell walls (0.3%). Interestingly, a significant difference was observed in the relative fatty-acid compositions between these two types of cell walls. Among the major fatty acids, there were increased concentrations of tetradecanoic acid (C14:0), hexadecanoic acid (C16:0), 9-hexadecenoic acid (C16: 1,Δ9), and 9-octadecanoic acid (C18:1,Δ9) and a concomitant decrease in 9,12-octadecadienoic acid (C18:2,Δ9,12) in less-pigmented compared with pigmented cell walls. This difference in fatty-acid composition may be related to the higher percentage of melanin in the pigmented than the less-pigmented cell walls. Lesser amounts of 9,12-octadecadienoic acid in less-pigmented cell walls may have been caused by the growth of the fungus under environmental stress conditions. An interesting observation was the presence in pigmented cell walls only of methyl-substituted fatty acids with carbon numbers C14 to C17, but their occurrence could not be ascertained in the present study.
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Literature Cited
Agarwal A, Garg GK, Devi S, Mishra DP, Singh US (1997) Ultrastructural changes in Brassica leaves caused by Alternaria brassicae and destruxin B. J Plant Physiol 106:1085–1093
Alviano CS, Farbiarz SR, De Souza W, Angluster J, Travassos LR (1991) Characterization of Fonsecaea pedrosoi melanin. J Gen Microbiol 137:837–844
Bell AA, Wheeler MH (1986) Biosynthesis and functions of fungal melanins. Annu Rev Phytopathol 24:411–451
Butler MJ, Day AW (1998) Fungal melanins: A review. Can J Microbiol 14:1115–1136
Calvo AM, Hinze LL, Gardner HW, Keller NP (1999) Sporogenic effect of polyunsaturated fatty acids on development of Aspergillus spp. Appl Environ Microbiol 65:3668–3673
Calvo AM, Wilson RA, Bok JW, Keller NP (2002) Relationship between metabolism and fungal development. Microbiol Mol Biol Rev 66:447–459
Chaplin MF (1986) Monosaccharides. In: Chaplin MF, Kennedy JF (eds) Carbohydrate analysis, a practical approach. Oxford, UK: IRL Press, pp 1–36
Fernell WR, King HK (1953). The simultaneous determination of pentose and hexose in mixtures of sugars. Analyst 78:80–83
Kanjilal S, Prasad RBN, Kaimal TNB, Ghafoorunissa Rao SH (1999) Synthesis and estimation of calorific value of a structured lipid-potential reduced calorie fat. Lipid 34:1045–1055
Kawamura C, Tsujimoto T, Tsuge T (1999) Targeted disruption of a melanin biosynthesis gene affects conidial development and UV tolerance in the Japanese pear pathotype of Alternaria alternata. Mol Plant Microbe Interact 12:59–63
Kimura N, Tsuge T (1993) Gene cluster involved in melanin biosynthesis of filamentous fungus Alternaria alternata. J Bacteriol 175:4427–4435
Pupin AM, Messias CJ, Piedrabuena AE, Roberts DW (2000) Total lipids and fatty acids of strains of Matarhizium anisopliae. Braz J Microbiol 31:121–128
Reddy RC, Misra S, Chandrashekhariah (2002) Alternaria tenuissima—A new fungal pathogen on mulberry stem. Indian Phytopathol 55:532–533
Shan R (1966) The polyunsaturated fatty acids of microorganisms. Adv Lipid Res 4:107–174
Thomma BPHJ (2003) Alternaria spp.: From general saprophyte to specific parasite. Mol Plant Pathol 4:225–236
Turian G, Bianchi DE (1972) Conidiation in Neurospora. Bot Ver 38:119–154
Van Etten JL, Gottlies D (1965) Biochemical changes during the growth of fungi. II. Ergosterol and fatty acids in Penicillium atrovenetum. J Bacteriol 89:409–414
Wang H, Li J, Bostock RM, Glichrist DG (1996) Apoptosis: A functional paradigm for programmed plant cell death induced by a host-selective phytotoxin and invoked during development. Plant Cell 8:375–391
Weete JD (1980) Lipid biochemistry of fungi and others organisms. New York, NY: Plenum, p. 388
Weete JD (1981) Lipids in fungal growth and reproduction. In: Turian G, Hohl HR (eds) The fungal spore: Morphogenic controls. New York, NY: Academic, pp 463–485
Weete JD, Lawer GC, Laseter JL (1973) Total lipid and esters components of Rhizopus arrhinius: Identification and metabolism. Arch Biochem Biophys 155:411–419
Wheeler MH, Bell AA (1988) Melanins and their importance in pathogenic fungi. Curr Top Med Mycol 2:338–387
Yemm EW, Cocking EC (1955) The determination of amino acids with ninhydrin. Analyst 80:209–213
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The authors are thankful to the director of the Indian Institute of Chemical Technology, Hyderabad, India for encouragement and support.
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Kishore, K.H., Kanjilal, S., Misra, S. et al. Comparative Chemical Characterization of Pigmented and Less Pigmented Cell Walls of Alternaria tenuissima. Curr Microbiol 51, 399–401 (2005). https://doi.org/10.1007/s00284-005-0098-7
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DOI: https://doi.org/10.1007/s00284-005-0098-7